Multiplexed detection of SARS-CoV-2 and other respiratory infections in high throughput by SARSeq

R Yelagandula, A Bykov, A Vogt, R Heinen… - Nature …, 2021 - nature.com
The COVID-19 pandemic has demonstrated the need for massively-parallel, cost-effective
tests monitoring viral spread. Here we present SARSeq, saliva analysis by RNA sequencing …

Tiled-ClickSeq for targeted sequencing of complete coronavirus genomes with simultaneous capture of RNA recombination and minority variants

E Jaworski, RM Langsjoen, B Mitchell, B Judy… - Elife, 2021 - elifesciences.org
High-throughput genomics of SARS-CoV-2 is essential to characterize virus evolution and to
identify adaptations that affect pathogenicity or transmission. While single-nucleotide …

Mini-XT, a miniaturized tagmentation-based protocol for efficient sequencing of SARS-CoV-2

M Fuchs, C Radulescu, M Tang, A Mahesh… - Journal of Translational …, 2022 - Springer
Background The COVID-19 pandemic has highlighted the importance of whole genome
sequencing (WGS) of SARS-CoV-2 to inform public health policy. By enabling definition of …

SARSeq, a robust and highly multiplexed NGS assay for parallel detection of SARS-CoV2 and other respiratory infections

R Yelagandula, A Bykov, A Vogt, R Heinen, E Özkan… - medRxiv, 2020 - medrxiv.org
During a pandemic, mitigation as well as protection of system-critical or vulnerable
institutions requires massively parallel, yet cost-effective testing to monitor the spread of …

Diagnostic applications for RNA-Seq technology and transcriptome analyses in human diseases caused by RNA viruses

IVS Louis, A Gorzalski, M Pandori - … of RNA-Seq in Biology and …, 2021 - books.google.com
Human diseases caused by single-stranded, positive-sense RNA viruses, are among the
deadliest of the 21st Century. In particular, there are two notable standouts: human …

Retrospective detection of SARS-CoV-2 in symptomatic patients prior to widespread diagnostic testing in Southern California

EE Hilt, J Boocock, M Trejo, CQ Le… - … Publication of the …, 2021 - pmc.ncbi.nlm.nih.gov
Background Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused
one of the worst pandemics in recent history. Few reports have revealed that SARS-CoV-2 …

[HTML][HTML] High-throughput sequencing approaches applied to SARS-CoV-2

EM Ong'era, KS Mohammed… - Wellcome Open …, 2023 - wellcomeopenresearch.org
High-throughput sequencing is crucial for surveillance and control of viral outbreaks. During
the ongoing coronavirus disease 2019 (COVID-19) pandemic, advances in the high …

McQ–An open-source multiplexed SARS-CoV-2 quantification platform

SC Vonesch, D Bredikhin, N Dobrev, L Villacorta… - medRxiv, 2020 - medrxiv.org
McQ is a SARS-CoV-2 quantification assay that couples early-stage barcoding with high-
throughput sequencing to enable multiplexed processing of thousands of samples. McQ is …

Retrospective detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in symptomatic patients prior to widespread diagnostic testing in southern …

EE Hilt, J Boocock, M Trejo, CQ Le… - Clinical Infectious …, 2022 - academic.oup.com
Background Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused
one of the worst pandemics in recent history. Few reports have revealed that SARS-CoV-2 …

Genomic epidemiology of the Los Angeles COVID-19 outbreak

L Guo, J Boocock, EE Hilt, S Chandrasekaran, Y Zhang… - medRxiv, 2020 - medrxiv.org
Abstract Los Angeles (LA) County has sustained a large outbreak of severe acute
respiratory syndrome coronavirus 2 (SARS-CoV-2). To learn about the transmission history …