Production of Industrial Enzymes via Pichia pastoris as a Cell Factory in Bioreactor: Current Status and Future Aspects

ZE Duman-Özdamar, B Binay - The protein journal, 2021 - Springer
Industrial enzymes have been widely preferred in various industries such as chemical
production, food & beverage, pharmaceutical, textile, cosmetics, etc. due to the …

Process control and optimization for heterologous protein production by methylotrophic Pichia pastoris

GAO Minjie, SHI Zhongping - Chinese Journal of Chemical Engineering, 2013 - Elsevier
The methylotrophic yeast Pichia pastoris is a highly successful system for production of a
variety of heterologous proteins due to its unique features/abilities for effective protein …

Non-repressing carbon sources for alcohol oxidase (AOX1) promoter of Pichia pastoris

M Inan, MM Meagher - Journal of Bioscience and Bioengineering, 2001 - Elsevier
The growth of Pichia pastoris in a mixture of either glycerol or glucose and methanol follows
a diauxic growth, with C1 utilizing enzymes being repressed. Therefore, these carbon …

Effect of copy number on the expression levels of hepatitis B surface antigen in the methylotrophic yeast Pichia pastoris

A Vassileva, DA Chugh, S Swaminathan… - Protein Expression and …, 2001 - Elsevier
High-level expression and efficient assembly of hepatitis B surface Antigen (HBsAg) has
been reported in Pichia pastoris by integrating a single copy of the HBsAg gene under …

Fermentation strategies for recombinant protein expression in the methylotrophic yeastPichia pastoris

W Zhang, M Inan, MM Meagher - Biotechnology and Bioprocess …, 2000 - Springer
Fermentation strategies for recombinant protein production in Pichia pastoris have been
investigated and are reviewed here. Characteristics of the expression system, such as …

Heterotrophic high cell‐density fed‐batch cultures of the phycocyanin‐producing red alga Galdieria sulphuraria

RA Schmidt, MG Wiebe… - Biotechnology and …, 2005 - Wiley Online Library
Growth and phycocyanin production in batch and fed‐batch cultures of the microalga
Galdieria sulphuraria 074G, which was grown heterotrophically in darkness on glucose …

Simple high-cell density fed-batch technique for high-level recombinant protein production with Pichia pastoris: Application to intracellular production of Hepatitis B …

C Gurramkonda, A Adnan, T Gäbel, H Lünsdorf… - Microbial Cell …, 2009 - Springer
Background Hepatitis B is a serious global public health concern. Though a safe and
efficacious recombinant vaccine is available, its use in several resource-poor countries is …

Effect of methanol feeding strategies on production and yield of recombinant mouse endostatin from Pichia pastoris

LB Trinh, JN Phue, J Shiloach - Biotechnology and …, 2003 - Wiley Online Library
Pichia pastoris, a methylotrophic yeast, is an efficient producer of recombinant proteins in
which the heterologous gene is under the control of the methanol‐induced AOX1 promoter …

Over expression of anti-MUC1 single-domain antibody fragments in the yeast Pichia pastoris

F Rahbarizadeh, MJ Rasaee, M Forouzandeh… - Molecular …, 2006 - Elsevier
The methylotrophic yeast Pichia pastoris has become a highly popular expression host
system for the recombinant production of a wide variety of proteins, such as antibody …

Novel transcriptional regulation of the GAP promoter in Pichia pastoris towards high expression of heterologous proteins

X Lin, W Ding, S Zheng, L Wu, X Chen, C Xie… - Microbial Cell …, 2024 - Springer
Abstract Background Pichia pastoris (Komagataella phaffii) is a promising production host,
but the usage of methanol limits its application in the medicine and food industries. Results …