Abstract One-carbon (C1) substrates are preferred feedstocks for the biomanufacturing
industry and have recently gained attention owing to their natural abundance, low …

Highlights•One-carbon compounds can be made from CO 2 to provide renewable microbial
feedstocks.•Methanol and formate have key advantages over gaseous one-carbon …

Methanol-based biorefinery is a promising strategy to achieve carbon neutrality goals by
linking CO2 capture and solar energy storage. As a typical methylotroph, Pichia pastoris …

Methanol, being electron rich and derivable from methane or CO 2, is a potentially
renewable one-carbon (C1) feedstock for microorganisms. Although the ribulose …

Methanol is a liquid with high energy storage capacity that holds promise as an alternative
substrate to replace sugars in the biotechnology industry. It can be produced from CO2 or …

Amino acid fermentation is one of the major pillars of industrial biotechnology. The multi-
billion USD amino acid market is rising steadily and is diversifying. Metabolic engineering is …

Carbon fixation is one of the most important biochemical processes. Most natural carbon
fixation pathways are thought to have emerged from enzymes that originally performed other …

Adaptive laboratory evolution (ALE) has served as a historic microbial engineering method
that mimics natural selection to obtain desired microbes. The past decade has witnessed …

The application of rational design in reallocating metabolic flux to accumulate desired
chemicals is always restricted by the native regulatory network. In this study, recombinant …

Methane is a promising feedstock with high abundance and low cost for the sustainable
production of biochemicals and biofuels. Methanotrophic bacteria are particularly interesting …