Myriad DNA-binding proteins undergo dynamic assembly, translocation, and conformational
changes while on DNA or alter the physical configuration of the DNA substrate to control its …

Topoisomerases are essential enzymes that regulate DNA topology. Type 1A family
topoisomerases are found in nearly all living organisms and are unique in that they require …

摘要光镊采用聚焦的激光束束缚微米, 纳米级的粒子, 具有亚皮牛级的力分辨率和亚毫秒级的
时间响应, 在单分子生物物理中具有广泛的应用. 通过化学耦链将生物大分子连接到高分子微球 …

In a previous paper (Syrjänen et al., 2014), we reported the first structural characterisation of
a synaptonemal complex (SC) protein, SYCP3, which led us to propose a model for its role …

Background DNA bridging promoted by the H-NS protein, combined with the compaction
induced by cellular crowding, plays a major role in the structuring of the E. coli genome …

The replisome is the multiprotein molecular machinery that replicates DNA. The replisome
components work in precise coordination to unwind the double helix of the DNA and …

Recent advances in the design and measurement capabilities of optical tweezers
instruments, and especially the combination with multi-color fluorescence detection, have …

Over the last few decades, in vitro single-molecule manipulation techniques have enabled
the use of force and displacement as controlled variables in biochemistry. Measuring the …

In optical trapping, whether a particle could be stably trapped into the focus region greatly
depends on the strength of the gradient force. Individual theoretical study on gradient force …

Biological macromolecules undergo dynamic conformational changes. Single-molecule
methods can track such structural rearrangements in real time. However, while the structure …