Native mass spectrometry (MS) involves the analysis and characterization of
macromolecules, predominantly intact proteins and protein complexes, whereby as much as …

We discuss the evolution of OrbitrapTM mass spectrometry (MS) from its birth in the late
1990s to its current role as one of the most prominent techniques for MS. The Orbitrap mass …

Native mass spectrometry (nMS) is evolving into a workhorse for structural biology. The
plethora of online and offline preparation, separation, and purification methods as well as …

Investigation of the structure, assembly and function of protein–nucleic acid macromolecular
machines requires multidimensional molecular and structural biology approaches. We …

Therapeutic monoclonal antibodies (mAbs) are glycoproteins produced by living cell
systems. The glycan moieties attached to the proteins can directly affect protein stability …

The emergence of proteomics has led to major technological advances in mass
spectrometry (MS). These advancements not only benefitted MS‐based high‐throughput …

Revised and updated, Chemical Reagents for Protein Modification, Third Edition is an
encyclopedic work describing the many approaches to the site-specific modification of …

Charge variant analysis (CVA) of monoclonal antibodies (mAbs) using cation exchange
chromatography is routinely used as a fingerprint of the distribution of posttranslational …

Advances in nuclease-based gene-editing technologies have enabled precise, stable, and
systematic genetic engineering of glycosylation capacities in mammalian cells, opening up a …

Many biopharmaceutical products exhibit extensive structural micro-heterogeneity due to an
array of co-occurring post-translational modifications. These modifications often effect the …