Kinetic mechanism of DNA polymerases: contributions of conformational dynamics and a third divalent metal ion
Faithful transmission and maintenance of genetic material is primarily fulfilled by DNA
polymerases. During DNA replication, these enzymes catalyze incorporation of …
polymerases. During DNA replication, these enzymes catalyze incorporation of …
Functional insights revealed by the kinetic mechanism of CRISPR/Cas9
The discovery of prokaryotic adaptive immunity prompted widespread use of the RNA-
guided clustered regularly interspaced short palindromic repeat (CRISPR)-associated (Cas) …
guided clustered regularly interspaced short palindromic repeat (CRISPR)-associated (Cas) …
Processing oxidatively damaged bases at DNA strand breaks by APE1
AM Whitaker, WJ Stark… - Nucleic Acids Research, 2022 - academic.oup.com
Reactive oxygen species attack the structure of DNA, thus altering its base-pairing
properties. Consequently, oxidative stress-associated DNA lesions are a major source of the …
properties. Consequently, oxidative stress-associated DNA lesions are a major source of the …
Beyond the Lesion: Back to High Fidelity DNA Synthesis
JD Kaszubowski, MA Trakselis - Frontiers in Molecular Biosciences, 2022 - frontiersin.org
High fidelity (HiFi) DNA polymerases (Pols) perform the bulk of DNA synthesis required to
duplicate genomes in all forms of life. Their structural features, enzymatic mechanisms, and …
duplicate genomes in all forms of life. Their structural features, enzymatic mechanisms, and …
Differential mechanisms of tolerance to extreme environmental conditions in tardigrades
D Carrero, JG Pérez-Silva, V Quesada, C López-Otín - Scientific reports, 2019 - nature.com
Tardigrades, also known as water bears, are small aquatic animals that inhabit marine, fresh
water or limno-terrestrial environments. While all tardigrades require surrounding water to …
water or limno-terrestrial environments. While all tardigrades require surrounding water to …
[HTML][HTML] Conformational transitions of Streptococcus pyogenes Cas9 induced by salt and single-guide RNA binding
Y He, N Zalenski, AA Stephenson, AT Raper… - Journal of Biological …, 2024 - Elsevier
ABSTRACT Streptococcus pyogenes (Sp) Cas9 has been widely utilized to edit genomes
across diverse species. To achieve high efficiency and specificity as a gene editing enzyme …
across diverse species. To achieve high efficiency and specificity as a gene editing enzyme …
Investigating the trade-off between folding and function in a multidomain Y-family DNA polymerase
The way in which multidomain proteins fold has been a puzzling question for decades. Until
now, the mechanisms and functions of domain interactions involved in multidomain protein …
now, the mechanisms and functions of domain interactions involved in multidomain protein …
Investigation of sliding DNA clamp dynamics by single-molecule fluorescence, mass spectrometry and structure-based modeling
Proliferating cell nuclear antigen (PCNA) is a trimeric ring-shaped clamp protein that
encircles DNA and interacts with many proteins involved in DNA replication and repair …
encircles DNA and interacts with many proteins involved in DNA replication and repair …
Investigation of intradomain motions of a Y-family DNA polymerase during substrate binding and catalysis
DNA polymerases catalyze DNA synthesis through a stepwise kinetic mechanism that
begins with binding to DNA, followed by selection, binding, and incorporation of a nucleotide …
begins with binding to DNA, followed by selection, binding, and incorporation of a nucleotide …
Influence of DNA lesions on polymerase-mediated DNA replication at single-molecule resolution
Faithful replication of DNA is a critical aspect in maintaining genome integrity. DNA
polymerases are responsible for replicating DNA, and high-fidelity polymerases do this …
polymerases are responsible for replicating DNA, and high-fidelity polymerases do this …