Nanopore adaptive sampling: a tool for enrichment of low abundance species in metagenomic samples

S Martin, D Heavens, Y Lan, S Horsfield, MD Clark… - Genome biology, 2022 - Springer
Adaptive sampling is a method of software-controlled enrichment unique to nanopore
sequencing platforms. To test its potential for enrichment of rarer species within …

DpCoA tagSeq: barcoding dpCoA-Capped RNA for direct nanopore sequencing via maleimide-thiol reaction

X Shao, H Zhang, Z Zhu, F Ji, Z He, Z Yang… - Analytical …, 2023 - ACS Publications
Recent discoveries of noncanonical RNA caps, such as nicotinamide adenine dinucleotide
(NAD+) and 3′-dephospho-coenzyme A (dpCoA), have expanded our knowledge of RNA …

Telomere‐to‐telomere Schizosaccharomyces japonicus genome assembly reveals hitherto unknown genome features

GJ Etherington, PS Wu, S Oliferenko, F Uhlmann… - Yeast, 2024 - Wiley Online Library
Schizosaccharomyces japonicus belongs to the single‐genus class Schizosaccharomycetes
, otherwise known as “fission yeasts.” As part of a composite model system with its widely …

High-Quality Genome Assembly of Fusarium oxysporum f. sp. lini

GS Krasnov, EN Pushkova, RO Novakovskiy… - Frontiers in …, 2020 - frontiersin.org
In the present work, a highly pathogenic isolate of Fusarium oxysporum f. sp. lini, which is
the most harmful pathogen affecting flax (Linum usitatissimum L.), was sequenced for the …

Measuring air metagenomic diversity in an agricultural ecosystem

M Giolai, W Verweij, S Martin, N Pearson, P Nicholson… - Current Biology, 2024 - cell.com
All species shed DNA during life or in death, providing an opportunity to monitor biodiversity
via environmental DNA (eDNA). In recent years, combining eDNA, high-throughput …

Multiple Displacement Amplification Facilitates SMRT Sequencing of Microscopic Animals and the Genome of the Gastrotrich Lepidodermella squamata (Dujardin …

NG Roberts, MJ Gilmore, TH Struck… - Genome Biology and …, 2024 - academic.oup.com
Obtaining adequate DNA for long-read genome sequencing remains a roadblock to
producing contiguous genomes from small-bodied organisms, hindering understanding of …

Microsatellite break-induced replication generates highly mutagenized extrachromosomal circular DNAs

RY Gadgil, SD Rider Jr, R Shrestha, V Alhawach… - NAR …, 2024 - academic.oup.com
Extrachromosomal circular DNAs (eccDNAs) are produced from all regions of the eucaryotic
genome. We used inverse PCR of non-B microsatellites capable of forming hairpin, triplex …

How low can you go? Driving down the DNA input requirements for nanopore sequencing

D Heavens, D Chooneea, M Giolai, P Cuber… - BioRxiv, 2021 - biorxiv.org
The requirement for large amounts of purified DNA limits many sequencing experiments,
especially when seeking to avoid pre-amplification or when using third generation …

[HTML][HTML] Detection and identification of authorized and unauthorized GMOs using high-throughput sequencing with the support of a sequence-based GMO database

A Saltykova, J Van Braekel, N Papazova… - Food Chemistry …, 2022 - Elsevier
The increasing number and diversity of genetically modified organisms (GMOs) for the food
and feed market calls for the development of advanced methods for their detection and …

Characterization and simulation of metagenomic nanopore sequencing data with Meta-NanoSim

C Yang, T Lo, KM Nip, S Hafezqorani, RL Warren… - …, 2023 - academic.oup.com
Background Nanopore sequencing is crucial to metagenomic studies as its kilobase-long
reads can contribute to resolving genomic structural differences among microbes. However …