Principles, applications, and biosafety of plant genome editing using CRISPR-Cas9
K El-Mounadi, ML Morales-Floriano… - Frontiers in plant …, 2020 - frontiersin.org
The terms genome engineering, genome editing, and gene editing, refer to modifications
(insertions, deletions, substitutions) in the genome of a living organism. The most widely …
(insertions, deletions, substitutions) in the genome of a living organism. The most widely …
[HTML][HTML] Genome editing in maize directed by CRISPR–Cas9 ribonucleoprotein complexes
S Svitashev, C Schwartz, B Lenderts, JK Young… - Nature …, 2016 - nature.com
Targeted DNA double-strand breaks have been shown to significantly increase the
frequency and precision of genome editing. In the past two decades, several double-strand …
frequency and precision of genome editing. In the past two decades, several double-strand …
Targeted mutagenesis, precise gene editing, and site-specific gene insertion in maize using Cas9 and guide RNA
S Svitashev, JK Young, C Schwartz, H Gao… - Plant …, 2015 - academic.oup.com
Targeted mutagenesis, editing of endogenous maize (Zea mays) genes, and site-specific
insertion of a trait gene using clustered regularly interspaced short palindromic repeats …
insertion of a trait gene using clustered regularly interspaced short palindromic repeats …
High-frequency, precise modification of the tomato genome
Background The use of homologous recombination to precisely modify plant genomes has
been challenging, due to the lack of efficient methods for delivering DNA repair templates to …
been challenging, due to the lack of efficient methods for delivering DNA repair templates to …
Gene replacements and insertions in rice by intron targeting using CRISPR–Cas9
J Li, X Meng, Y Zong, K Chen, H Zhang, J Liu, J Li… - Nature plants, 2016 - nature.com
Sequence-specific nucleases have been exploited to create targeted gene knockouts in
various plants 1, but replacing a fragment and even obtaining gene insertions at specific loci …
various plants 1, but replacing a fragment and even obtaining gene insertions at specific loci …
Cas9-guide RNA directed genome editing in soybean
Z Li, ZB Liu, A Xing, BP Moon, JP Koellhoffer… - Plant …, 2015 - academic.oup.com
Recently discovered bacteria and archaea adaptive immune system consisting of clustered
regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) …
regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) …
First Report of CRISPR/Cas9 Mediated DNA-Free Editing of 4CL and RVE7 Genes in Chickpea Protoplasts
The current genome editing system Clustered Regularly Interspaced Short Palindromic
Repeats Cas9 (CRISPR/Cas9) has already confirmed its proficiency, adaptability, and …
Repeats Cas9 (CRISPR/Cas9) has already confirmed its proficiency, adaptability, and …
Both CRISPR/Cas‐based nucleases and nickases can be used efficiently for genome engineering in Arabidopsis thaliana
Engineered nucleases can be used to induce site‐specific double‐strand breaks (DSB s) in
plant genomes. Thus, homologous recombination (HR) can be enhanced and targeted …
plant genomes. Thus, homologous recombination (HR) can be enhanced and targeted …
Repair of strand breaks by homologous recombination
M Jasin, R Rothstein - Cold Spring Harbor perspectives …, 2013 - cshperspectives.cshlp.org
In this review, we discuss the repair of DNA double-strand breaks (DSBs) using a
homologous DNA sequence (ie, homologous recombination [HR]), focusing mainly on yeast …
homologous DNA sequence (ie, homologous recombination [HR]), focusing mainly on yeast …