The fluorescent protein palette: tools for cellular imaging

RN Day, MW Davidson - Chemical Society Reviews, 2009 - pubs.rsc.org
This critical review provides an overview of the continually expanding family of fluorescent
proteins (FPs) that have become essential tools for studies of cell biology and physiology …

Fluorescence lifetime imaging microscopy in life sciences

JW Borst, AJWG Visser - Measurement Science and Technology, 2010 - iopscience.iop.org
Fluorescence lifetime imaging microscopy (FLIM) and fluorescence anisotropy imaging
microscopy (FAIM) are versatile tools for the investigation of the molecular environment of …

[图书][B] FRET-Förster resonance energy transfer: from theory to applications

IL Medintz, N Hildebrandt - 2013 - books.google.com
FRET–Förster Resonance Energy Transfer Meeting the need for an up-to-date and detailed
primer on all aspects of the topic, this ready reference reflects the incredible expansion in …

The macrophage tetraspan MS4A4A enhances dectin-1-dependent NK cell–mediated resistance to metastasis

I Mattiola, F Tomay, M De Pizzol, R Silva-Gomes… - Nature …, 2019 - nature.com
The plasma membrane tetraspan molecule MS4A4A is selectively expressed by
macrophage-lineage cells, but its function is unknown. Here we report that MS4A4A was …

Involvement of the LSPR spectral overlap for energy transfer between a dye and Au nanoparticle

MP Singh, GF Strouse - Journal of the American Chemical Society, 2010 - ACS Publications
Nanometal surface energy transfer (NSET) is a molecular ruler technique that has been
utilized to optically probe long distances in biomolecular structures. We investigate the …

Quantitative FRET Analysis With the E0GFP‐mCherry Fluorescent Protein Pair

L Albertazzi, D Arosio, L Marchetti… - Photochemistry and …, 2009 - Wiley Online Library
Fluorescence resonance energy transfer (FRET) between fluorescent proteins (FPs) is a
powerful tool to investigate protein–protein interaction and even protein modifications in …

Recent advances using green and red fluorescent protein variants

A Müller-Taubenberger, KI Anderson - Applied microbiology and …, 2007 - Springer
Fluorescent proteins have proven to be excellent tools for live-cell imaging. In addition to
green fluorescent protein (GFP) and its variants, recent progress has led to the development …

Rational design of photoconvertible and biphotochromic fluorescent proteins for advanced microscopy applications

V Adam, B Moeyaert, CC David, H Mizuno… - Chemistry & biology, 2011 - cell.com
Advanced fluorescence imaging, including subdiffraction microscopy, relies on fluorophores
with controllable emission properties. Chief among these fluorophores are the …

De novo phosphorylation and conformational opening of the tyrosine kinase Lck act in concert to initiate T cell receptor signaling

L Philipsen, AV Reddycherla, R Hartig, J Gumz… - Science …, 2017 - science.org
The enzymatic activity of the Src family tyrosine kinase p56Lck (Lck) is tightly controlled by
differential phosphorylation of two tyrosine residues, Tyr394 and Tyr505. Phosphorylation of …

T cell activation results in conformational changes in the Src family kinase Lck to induce its activation

A Stirnweiss, R Hartig, S Gieseler, JA Lindquist… - Science …, 2013 - science.org
The lymphocyte-specific Src family protein tyrosine kinase p56Lck (Lck) is essential for T cell
development and activation and, hence, for adaptive immune responses. The mechanism by …