Native chemical ligation (NCL) provides a powerful solution to assemble proteins with
precise chemical features, which enables a detailed investigation of the protein structure …

The hydrazinolysis of S‐cyanylated peptide provides an alternative way to afford protein α‐
hydrazide, a key reagent used in native chemical ligation (NCL), without the aid of any …

We report here a robust and practical strategy for chemical protein synthesis using an o-
nitrobenzyl group as a temporary protective group for an N-terminal cysteine residue of …

C‐terminal hydrazides are an important class of synthetic peptides with an ever expanding
scope of applications, but their widespread application for chemical protein synthesis has …

Total chemical synthesis of proteins offers both naturally occurring proteins and artificially
engineered proteins through single or multiple ligation reactions of synthetic peptide …

Microtubules are natural tubular protein assemblies consisting of tubulin dimers, which
serve various roles in cells as cytoskeletons. Because of their unique properties …

Peptide ligation chemistries have revolutionized the synthesis of proteins with site-specific
modifications or proteomimetics through assembly of multiple peptide segments. In order to …