Super-resolution microscopy techniques, and specifically single-molecule localization
microscopy (SMLM), are approaching nanometer resolution inside cells and thus have great …

Structural biologists have traditionally approached cellular complexity in a reductionist
manner in which the cellular molecular components are fractionated and purified before …

INTRODUCTION The eukaryotic nucleus protects the genome and is enclosed by the two
membranes of the nuclear envelope. Nuclear pore complexes (NPCs) perforate the nuclear …

INTRODUCTION The nucleus harbors and protects the genetic information in eukaryotes. It
is surrounded by the nuclear envelope, which separates the nucleoplasm from the cytosol …

INTRODUCTION The nuclear pore complex (NPC) is the molecular conduit in the nuclear
membrane of eukaryotic cells that regulates import and export of biomolecules between the …

Nuclear pore complexes (NPCs) create large conduits for cargo transport between the
nucleus and cytoplasm across the nuclear envelope (NE),–. These multi-megadalton …

The ultimate goal of biological super-resolution fluorescence microscopy is to provide three-
dimensional resolution at the size scale of a fluorescent marker. Here we show that by …

Summary Human immunodeficiency virus (HIV-1) remains a major health threat. Viral capsid
uncoating and nuclear import of the viral genome are critical for productive infection. The …

The nuclear pore complex (NPC) serves as the sole bidirectional gateway of
macromolecules in and out of the nucleus. Owing to its size and complexity (∼ 1,000 protein …

INTRODUCTION Cytoplasmic face of the human NPC. Near-atomic composite structure of
the NPC generated by docking high-resolution crystal structures into a cryo‑ET …