Since the advent of induced pluripotent stem cell (iPSC) technology a decade ago,
enormous progress has been made in stem cell biology and regenerative medicine. Human …

Attempts to enhance human memory and learning ability have a long tradition in science.
This topic has recently gained substantial attention because of the increasing percentage of …

Transcription factors (TFs) regulate gene programs, thereby controlling diverse cellular
processes and cell states. To comprehensively understand TFs and the programs they …

Prolonged expression of the CRISPR-Cas9 nuclease and gRNA from viral vectors may
cause off-target mutagenesis and immunogenicity. Thus, a transient delivery system is …

The generation of functional skeletal muscle tissues from human pluripotent stem cells
(hPSCs) has not been reported. Here, we derive induced myogenic progenitor cells (iMPCs) …

Duchenne muscular dystrophy (DMD) is a severe muscle-degenerative disease caused by a
mutation in the dystrophin gene. Genetic correction of patient-derived induced pluripotent …

Although single-component Class 2 CRISPR systems, such as type II Cas9 or type V
Cas12a (Cpf1), are widely used for genome editing in eukaryotic cells, the application of …

Progress toward finding a cure for muscle diseases has been slow because of the absence
of relevant cellular models and the lack of a reliable source of muscle progenitors for …

The induced pluripotent stem cell (i PSC) technology is instrumental in advancing the fields
of disease modeling and cell transplantation. We herein discuss the various issues …

During embryonic development, skeletal muscles arise from somites, which derive from the
presomitic mesoderm (PSM). Using PSM development as a guide, we establish conditions …