Highlights•Guidelines for in-house and inter-laboratory validation of qualitative qPCR
methods.•Method acceptance and performance parameters to be evaluated.•Acceptance …

The number of genetically modified organisms (GMOs) on the market is steadily increasing.
Because of regulation of cultivation and trade of GMOs in several countries, there is …

In many countries, genetically modified organisms (GMO) legislations have been
established in order to guarantee the traceability of food/feed products on the market and to …

The advantages of the digital PCR technology are already well documented until now. One
way to achieve better cost efficiency of the technique is to use it in a multiplexing strategy …

Presence of genetically modified organisms (GMO) in food and feed products is regulated in
many countries. The European Union (EU) has implemented a threshold for labeling of …

Genetically modified (GM) crops are the fastest adopted commodities in the agribiotech
industry. This market penetration should provide a sustainable basis for ensuring food …

Because the number and diversity of genetically modified (GM) crops has significantly
increased, their analysis based on real-time PCR (qPCR) methods is becoming increasingly …

Quantification of genetically modified organisms (GMOs) in food and feed products is often
required for their labelling or for tolerance thresholds. Standard-curve-based simplex …

DNA analysis of processed foods is performed widely to detect various targets, such as
genetically modified organisms (GMOs). Food processing often causes DNA fragmentation …

The proliferation of genetically modified organisms (GMOs) presents challenges to GMO
testing laboratories and policymakers. Traditional methods, like quantitative real-time PCR …