The use of next-generation sequencing (NGS) has revolutionized the way phenotypic traits
are assigned to genes. In this review, we describe NGS-based methods for mapping a …

Forward genetics is a powerful tool to unravel molecular mechanisms of diverse biological
processes. The success of genetic screens primarily relies on the ease of genetic …

Mutants remain a powerful means for dissecting gene function in model organisms such as
Caenorhabditis elegans. Massively parallel sequencing has simplified the detection of …

Whole-genome sequencing is a powerful tool for analyzing genetic variation on a global
scale. One particularly useful application is the identification of mutations obtained by …

Ciliary microtubules are subject to post-translational modifications that act as a “Tubulin
Code” to regulate motor traffic, binding proteins and stability. In humans, loss of CCP1, a …

Whole-genome sequencing provides a rapid and powerful method for identifying mutations
on a global scale, and has spurred a renewed enthusiasm for classical genetic screens in …

The production of viable embryos requires the coordination of many cellular processes,
including protein synthesis, cytoskeletal reorganization, establishment of polarity, cell …

After fertilization, rapid changes of the Caenorhabditis elegans cytoskeleton occur in the
transition from meiosis to mitosis, requiring precise regulation. The MEI-1/MEI-2 katanin …

Orthologous phenotypes, or phenologs, are seemingly unrelated phenotypes generated by
mutations in a conserved set of genes. Phenologs have been widely observed and accepted …

Centrioles play critical roles in the organization of microtubule-based structures, from the
mitotic spindle to cilia and flagella. In order to properly execute their various functions …