Glycosidases are ubiquitous enzymes that catalyze the hydrolysis of glycosidic linkages in
oligosaccharides and glycoconjugates. These enzymes play a vital role in a wide variety of …

Activity-based protein profiling (ABPP) is an effective technology for the identification and
functional annotation of enzymes in complex biological samples. ABP designs are normally …

Changing an oxygen atom of the phosphoester bond in phosphopeptides by a sulfur atom
enables instantly targeting Golgi apparatus (GA) and selectively killing cancer cells by …

The multifunctional human Parkinson's disease protein 7 (PARK7/DJ1) is an attractive
therapeutic target due to its link with early-onset Parkinson's disease, upregulation in …

Siastatin B is a potent and effective iminosugar inhibitor of three diverse glycosidase
classes, namely, sialidases, β-d-glucuronidases, and N-acetyl-glucosaminidases. The mode …

Amylases are key enzymes in the processing of starch in many kingdoms of life. They are
important catalysts in industrial biotechnology where they are applied in, among others, food …

Modulation of N-glycosylation using human Golgi α-mannosidase II (α-hGMII) inhibitors is a
potential anticancer approach, but the clinical utility of current α-hGMII inhibitors is limited by …

Highlights•Activity-based probes are versatile tools to study protein function and biological
context.•Selective and non-selective ABPs are used for target evaluation.•Proteomic …

The development of effective inhibitors of Golgi α-mannosidase II (GMII, EC 3.2. 1.114) with
minimal off-target effects on phylogenetically-related lysosomal α-mannosidase (LMan, EC …

This personal account focuses on synthesis of polyhydroxylated piperidines, a subset of
compounds within the iminosugar family. Cyclisations to form the piperidine ring include …