Recent advances in metabolic engineering of Saccharomyces cerevisiae: new tools and their applications
Metabolic engineering aims to develop efficient cell factories by rewiring cellular
metabolism. As one of the most commonly used cell factories, Saccharomyces cerevisiae …
metabolism. As one of the most commonly used cell factories, Saccharomyces cerevisiae …
Proteome-wide structural changes measured with limited proteolysis-mass spectrometry: an advanced protocol for high-throughput applications
Proteins regulate biological processes by changing their structure or abundance to
accomplish a specific function. In response to a perturbation, protein structure may be …
accomplish a specific function. In response to a perturbation, protein structure may be …
Global detection of human variants and isoforms by deep proteome sequencing
An average shotgun proteomics experiment detects approximately 10,000 human proteins
from a single sample. However, individual proteins are typically identified by peptide …
from a single sample. However, individual proteins are typically identified by peptide …
Six alternative proteases for mass spectrometry–based proteomics beyond trypsin
Protein digestion using a dedicated protease represents a key element in a typical mass
spectrometry (MS)-based shotgun proteomics experiment. Up to now, digestion has been …
spectrometry (MS)-based shotgun proteomics experiment. Up to now, digestion has been …
pGlyco 2.0 enables precision N-glycoproteomics with comprehensive quality control and one-step mass spectrometry for intact glycopeptide identification
The precise and large-scale identification of intact glycopeptides is a critical step in
glycoproteomics. Owing to the complexity of glycosylation, the current overall throughput …
glycoproteomics. Owing to the complexity of glycosylation, the current overall throughput …
SPOCD1 is an essential executor of piRNA-directed de novo DNA methylation
A Zoch, T Auchynnikava, RV Berrens, Y Kabayama… - Nature, 2020 - nature.com
In mammals, the acquisition of the germline from the soma provides the germline with an
essential challenge: the need to erase and reset genomic methylation. In the male germline …
essential challenge: the need to erase and reset genomic methylation. In the male germline …
Label-free quantification in ion mobility–enhanced data-independent acquisition proteomics
Unbiased data-independent acquisition (DIA) strategies have gained increased popularity in
the field of quantitative proteomics. The integration of ion mobility separation (IMS) into DIA …
the field of quantitative proteomics. The integration of ion mobility separation (IMS) into DIA …
Silicon: potential to promote direct and indirect effects on plant defense against arthropod pests in agriculture
OL Reynolds, MP Padula, R Zeng… - Frontiers in plant …, 2016 - frontiersin.org
Silicon has generally not been considered essential for plant growth, although it is well
recognized that many plants, particularly Poaceae, have substantial plant tissue …
recognized that many plants, particularly Poaceae, have substantial plant tissue …
Measuring protein structural changes on a proteome-wide scale using limited proteolysis-coupled mass spectrometry
S Schopper, A Kahraman, P Leuenberger, Y Feng… - Nature protocols, 2017 - nature.com
Protein structural changes induced by external perturbations or internal cues can profoundly
influence protein activity and thus modulate cellular physiology. A number of biophysical …
influence protein activity and thus modulate cellular physiology. A number of biophysical …
Phosphoproteomics in the age of rapid and deep proteome profiling
Protein phosphorylation is a post-translational modification (PTM) that orchestrates a diverse
array of cellular processes. Because this modification serves as a rapid and reversible …
array of cellular processes. Because this modification serves as a rapid and reversible …