Zinc-finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs)
comprise a powerful class of tools that are redefining the boundaries of biological research …

RNA-guided Cas9 nucleases derived from clustered regularly interspaced short palindromic
repeats (CRISPR)-Cas systems have dramatically transformed our ability to edit the …

Targeted nucleases have provided researchers with the ability to manipulate virtually any
genomic sequence, enabling the facile creation of isogenic cell lines and animal models for …

Current technology enables the production of highly specific genome modifications with
excellent efficiency and specificity. Key to this capability are targetable DNA cleavage …

We describe the development of 'recCas9', an RNA-programmed small serine recombinase
that functions in mammalian cells. We fused a catalytically inactive dCas9 to the catalytic …

Gene therapy by engineered nucleases is a genetic intervention being investigated for
curing the hereditary disorders by targeting selected genes with specific nucleotides for …

Conspectus The understanding of gene regulation and the structure and function of the
human genome increased dramatically at the end of the 20th century. Yet the technologies …

An increasing interest in building novel biological devices with designed cellular
functionalities has triggered the search of innovative tools for biocomputation. Utilizing the …

Human induced pluripotent stem cells (hiPSCs) have revolutionized the field of human
disease modeling, with an enormous potential to serve as paradigm shifting platforms for …

The fields of molecular genetics, biotechnology and synthetic biology are demanding ever
more sophisticated molecular tools for programmed precise modification of cell genomic …