Super-resolving microscopy in neuroscience
Fluorescence imaging techniques play a pivotal role in our understanding of the nervous
system. The emergence of various super-resolution microscopy methods and specialized …
system. The emergence of various super-resolution microscopy methods and specialized …
AAV targeting of glial cell types in the central and peripheral nervous system and relevance to human gene therapy
Different glial cell types are found throughout the central (CNS) and peripheral nervous
system (PNS), where they have important functions. These cell types are also involved in …
system (PNS), where they have important functions. These cell types are also involved in …
Visualization of cortical, subcortical and deep brain neural circuit dynamics during naturalistic mammalian behavior with head-mounted microscopes and chronically …
Genetically encoded calcium indicators for visualizing dynamic cellular activity have greatly
expanded our understanding of the brain. However, owing to the light-scattering properties …
expanded our understanding of the brain. However, owing to the light-scattering properties …
CRISPR/Cas9 mediated disruption of the Swedish APP allele as a therapeutic approach for early-onset Alzheimer's disease
The APPswe (Swedish) mutation in the amyloid precursor protein (APP) gene causes
dominantly inherited Alzheimer's disease (AD) as a result of increased β-secretase cleavage …
dominantly inherited Alzheimer's disease (AD) as a result of increased β-secretase cleavage …
Cellular selectivity of AAV serotypes for gene delivery in neurons and astrocytes by neonatal intracerebroventricular injection
SL Hammond, AN Leek, EH Richman, RB Tjalkens - PloS one, 2017 - journals.plos.org
The non-pathogenic parvovirus, adeno-associated virus (AAV), is an efficient vector for
transgene expression in vivo and shows promise for treatment of brain disorders in clinical …
transgene expression in vivo and shows promise for treatment of brain disorders in clinical …
Controlling AAV tropism in the nervous system with natural and engineered capsids
MJ Castle, HT Turunen, LH Vandenberghe… - Gene Therapy for …, 2016 - Springer
More than one hundred naturally occurring variants of adeno-associated virus (AAV) have
been identified, and this library has been further expanded by an array of techniques for …
been identified, and this library has been further expanded by an array of techniques for …
Transfection techniques for neuronal cells
D Karra, R Dahm - Journal of Neuroscience, 2010 - Soc Neuroscience
The transfection of nucleic acids into cells is crucial for the study of many aspects of
neuronal cell biology. These include investigating gene and protein function by knocking …
neuronal cell biology. These include investigating gene and protein function by knocking …
Parthanatos mediates AIMP2-activated age-dependent dopaminergic neuronal loss
Y Lee, SS Karuppagounder, JH Shin, YI Lee… - Nature …, 2013 - nature.com
The defining pathogenic feature of Parkinson's disease is the age-dependent loss of
dopaminergic neurons. Mutations and inactivation of parkin, an ubiquitin E3 ligase, induce …
dopaminergic neurons. Mutations and inactivation of parkin, an ubiquitin E3 ligase, induce …
Adeno-associated virus serotypes 1, 8, and 9 share conserved mechanisms for anterograde and retrograde axonal transport
Adeno-associated virus (AAV) vectors often undergo long-distance axonal transport after
brain injection. This leads to transduction of brain regions distal to the injection site, although …
brain injection. This leads to transduction of brain regions distal to the injection site, although …
Achieving high-frequency optical control of synaptic transmission
SL Jackman, BM Beneduce, IR Drew… - Journal of …, 2014 - Soc Neuroscience
The optogenetic tool channelrhodopsin-2 (ChR2) is widely used to excite neurons to study
neural circuits. Previous optogenetic studies of synapses suggest that light-evoked synaptic …
neural circuits. Previous optogenetic studies of synapses suggest that light-evoked synaptic …