Metagenomic approaches in microbial ecology: an update on whole-genome and marker gene sequencing analyses
AE Pérez-Cobas, L Gomez-Valero… - Microbial …, 2020 - microbiologyresearch.org
Metagenomics and marker gene approaches, coupled with high-throughput sequencing
technologies, have revolutionized the field of microbial ecology. Metagenomics is a culture …
technologies, have revolutionized the field of microbial ecology. Metagenomics is a culture …
Review and re-analysis of domain-specific 16S primers
GC Baker, JJ Smith, DA Cowan - Journal of microbiological methods, 2003 - Elsevier
The Polymerase Chain Reaction (PCR) has facilitated the detection of unculturable
microorganisms in virtually any environmental source and has thus been used extensively in …
microorganisms in virtually any environmental source and has thus been used extensively in …
Development of a Prokaryotic Universal Primer for Simultaneous Analysis of Bacteria and Archaea Using Next-Generation Sequencing
S Takahashi, J Tomita, K Nishioka, T Hisada… - PloS one, 2014 - journals.plos.org
For the analysis of microbial community structure based on 16S rDNA sequence diversity,
sensitive and robust PCR amplification of 16S rDNA is a critical step. To obtain accurate …
sensitive and robust PCR amplification of 16S rDNA is a critical step. To obtain accurate …
Deciphering microbial interactions and detecting keystone species with co-occurrence networks
Co-occurrence networks produced from microbial survey sequencing data are frequently
used to identify interactions between community members. While this approach has …
used to identify interactions between community members. While this approach has …
The variability of the 16S rRNA gene in bacterial genomes and its consequences for bacterial community analyses
T Větrovský, P Baldrian - PloS one, 2013 - journals.plos.org
16S ribosomal RNA currently represents the most important target of study in bacterial
ecology. Its use for the description of bacterial diversity is, however, limited by the presence …
ecology. Its use for the description of bacterial diversity is, however, limited by the presence …
The choice of universal primers and the characteristics of the species mixture determine when DNA metabarcoding can be quantitative
DNA metabarcoding is a technique used to survey biodiversity in many ecological settings,
but there are doubts about whether it can provide quantitative results, that is, the proportions …
but there are doubts about whether it can provide quantitative results, that is, the proportions …
[图书][B] Experimental methods in wastewater treatment
Over the past twenty years, the knowledge and understanding of wastewater treatment has
advanced extensively and moved away from empirically based approaches to a …
advanced extensively and moved away from empirically based approaches to a …
Incorporating 16S gene copy number information improves estimates of microbial diversity and abundance
The abundance of different SSU rRNA (“16S”) gene sequences in environmental samples is
widely used in studies of microbial ecology as a measure of microbial community structure …
widely used in studies of microbial ecology as a measure of microbial community structure …
Evaluation of methods for the extraction and purification of DNA from the human microbiome
Background DNA extraction is an essential step in all cultivation-independent approaches to
characterize microbial diversity, including that associated with the human body. A …
characterize microbial diversity, including that associated with the human body. A …
Determination of bacterial load by real-time PCR using a broad-range (universal) probe and primers set
MA Nadkarni, FE Martin, NA Jacques… - …, 2002 - microbiologyresearch.org
The design and evaluation of a set of universal primers and probe for the amplification of
16S rDNA from the Domain Bacteria to estimate total bacterial load by real-time PCR is …
16S rDNA from the Domain Bacteria to estimate total bacterial load by real-time PCR is …