Towards application of CRISPR-Cas12a in the design of modern viral DNA detection tools

J Dronina, U Samukaite-Bubniene… - Journal of …, 2022 - Springer
Early detection of viral pathogens by DNA-sensors in clinical samples, contaminated foods,
soil or water can dramatically improve clinical outcomes and reduce the socioeconomic …

CRISPR/Cas genome editing technologies for plant improvement against biotic and abiotic stresses: advances, limitations, and future perspectives

Y Wang, N Zafar, Q Ali, H Manghwar, G Wang, L Yu… - Cells, 2022 - mdpi.com
Crossbreeding, mutation breeding, and traditional transgenic breeding take much time to
improve desirable characters/traits. CRISPR/Cas-mediated genome editing (GE) is a game …

Single-cell transcriptomic analysis reveals the developmental trajectory and transcriptional regulatory networks of pigment glands in Gossypium bickii

Y Sun, Y Han, K Sheng, P Yang, Y Cao, H Li, QH Zhu… - Molecular Plant, 2023 - cell.com
Comprehensive utilization of cottonseeds is limited by the presence of pigment glands and
its inclusion gossypol. The ideal cotton has glandless seeds but a glanded plant, a trait …

Application of CRISPR/Cas9-mediated gene editing for abiotic stress management in crop plants

M Kumar, MR Prusty, MK Pandey, PK Singh… - Frontiers in plant …, 2023 - frontiersin.org
Abiotic stresses, including drought, salinity, cold, heat, and heavy metals, extensively
reducing global agricultural production. Traditional breeding approaches and transgenic …

Genetic manipulation of anti-nutritional factors in major crops for a sustainable diet in future

A Duraiswamy, NM Sneha A, S Jebakani K… - Frontiers in plant …, 2023 - frontiersin.org
The consumption of healthy food, in order to strengthen the immune system, is now a major
focus of people worldwide and is essential to tackle the emerging pandemic concerns …

Development of an efficient and precise adenine base editor (ABE) with expanded target range in allotetraploid cotton (Gossypium hirsutum)

G Wang, Z Xu, F Wang, Y Huang, Y Xin, S Liang, B Li… - BMC biology, 2022 - Springer
Abstract Background Base editors (BEs) display diverse applications in a variety of plant
species such as Arabidopsis, rice, wheat, maize, soybean, and cotton, where they have …

The dual role of GoPGF reveals that the pigment glands are synthetic sites of gossypol in aerial parts of cotton

ZN Zhang, L Long, XT Zhao, SZ Shang, FC Xu… - New …, 2024 - Wiley Online Library
Gossypol and the related terpenoids are stored in the pigment gland to protect cotton plants
from biotic stresses, but little is known about the synthetic sites of these metabolites. Here …

CRISPR/dCas13(Rx) Derived RNA N6‐methyladenosine (m6A) Dynamic Modification in Plant

L Yu, M Alariqi, B Li, A Hussain, H Zhou… - Advanced …, 2024 - Wiley Online Library
Abstract N6‐methyladenosine (m6A) is the most prevalent internal modification of mRNA
and plays an important role in regulating plant growth. However, there is still a lack of …

Large chromosomal segment deletions by CRISPR/LbCpf1‐mediated multiplex gene editing in soybean

K Duan, Y Cheng, J Ji, C Wang, Y Wei… - Journal of Integrative …, 2021 - Wiley Online Library
The creation of new soybean varieties has been limited by genomic duplication and
redundancy. Efficient multiplex gene editing and large chromosomal segment deletion …

Herbicide resistance: another hot agronomic trait for plant genome editing

A Hussain, X Ding, M Alariqi, H Manghwar, F Hui, Y Li… - Plants, 2021 - mdpi.com
Weeds have continually interrupted crop plants since their domestication, leading to a
greater yield loss compared to diseases and pests that necessitated the practice of weed …