Cryo-electron tomography: the challenge of doing structural biology in situ
Electron microscopy played a key role in establishing cell biology as a discipline, by
producing fundamental insights into cellular organization and ultrastructure. Many seminal …
producing fundamental insights into cellular organization and ultrastructure. Many seminal …
Macromolecular crowding, phase separation, and homeostasis in the orchestration of bacterial cellular functions
Macromolecular crowding affects the activity of proteins and functional macromolecular
complexes in all cells, including bacteria. Crowding, together with physicochemical …
complexes in all cells, including bacteria. Crowding, together with physicochemical …
Preparing samples from whole cells using focused-ion-beam milling for cryo-electron tomography
FR Wagner, R Watanabe, R Schampers, D Singh… - Nature protocols, 2020 - nature.com
Recent advances have made cryogenic (cryo) electron microscopy a key technique to
achieve near-atomic-resolution structures of biochemically isolated macromolecular …
achieve near-atomic-resolution structures of biochemically isolated macromolecular …
Electron tomography: a three‐dimensional analytic tool for hard and soft materials research
Three‐dimensional (3D) structural analysis is essential to understand the relationship
between the structure and function of an object. Many analytical techniques, such as X‐ray …
between the structure and function of an object. Many analytical techniques, such as X‐ray …
Insights into the structure, function, and dynamics of the bacterial cytokinetic FtsZ-ring
R McQuillen, J Xiao - Annual Review of Biophysics, 2020 - annualreviews.org
The FtsZ protein is a highly conserved bacterial tubulin homolog. In vivo, the functional form
of FtsZ is the polymeric, ring-like structure (Z-ring) assembled at the future division site …
of FtsZ is the polymeric, ring-like structure (Z-ring) assembled at the future division site …
Pushing and pulling in prokaryotic DNA segregation
K Gerdes, M Howard, F Szardenings - Cell, 2010 - cell.com
In prokaryotes, DNA can be segregated by three different types of cytoskeletal filaments. The
best-understood type of partitioning (par) locus encodes an actin homolog called ParM …
best-understood type of partitioning (par) locus encodes an actin homolog called ParM …
Plasmid partition mechanisms
JC Baxter, BE Funnell - Plasmids: biology and impact in …, 2015 - Wiley Online Library
The stable maintenance of low‐copy‐number plasmids in bacteria is actively driven by
partition mechanisms that are responsible for the positioning of plasmids inside the cell …
partition mechanisms that are responsible for the positioning of plasmids inside the cell …
Entropy as the driver of chromosome segregation
S Jun, A Wright - Nature Reviews Microbiology, 2010 - nature.com
We present a new physical biology approach to understanding the relationship between the
organization and segregation of bacterial chromosomes. We posit that replicated …
organization and segregation of bacterial chromosomes. We posit that replicated …
Synthesis of empty bacterial microcompartments, directed organelle protein incorporation, and evidence of filament-associated organelle movement
Compartmentalization is an important process, since it allows the segregation of metabolic
activities and, in the era of synthetic biology, represents an important tool by which defined …
activities and, in the era of synthetic biology, represents an important tool by which defined …
Cellular electron cryotomography: toward structural biology in situ
CM Oikonomou, GJ Jensen - Annual review of biochemistry, 2017 - annualreviews.org
Electron cryotomography (ECT) provides three-dimensional views of macromolecular
complexes inside cells in a native frozen–hydrated state. Over the last two decades, ECT …
complexes inside cells in a native frozen–hydrated state. Over the last two decades, ECT …