A strong connection exists between the cell cycle and mechanisms required for executing
cell fate decisions in a wide-range of developmental contexts. Terminal differentiation is …

For most genes, mRNA transcript abundance scales with cell size to ensure a constant
concentration. Scaling of mRNA synthesis rates with cell size plays an important role, with …

We perform a thorough analysis of RNA velocity methods, with a view towards
understanding the suitability of the various assumptions underlying popular …

Single-cell RNA sequencing (scRNA-seq) is generally used for profiling transcriptome of
individual cells. The droplet-based 10X Genomics Chromium (10X) approach and the plate …

Spatiotemporal regulation of the cellular transcriptome is crucial for proper protein
expression and cellular function. However, the intricate subcellular dynamics of RNA remain …

The stochasticity of gene expression presents significant challenges to the modeling of
genetic networks. A two-state model describing promoter switching, transcription, and …

Cell proliferation is a fundamental requirement for organismal development and
homeostasis. The mammalian cell division cycle is tightly controlled to ensure complete and …

RNA abundance is tightly regulated in eukaryotic cells by modulating the kinetic rates of
RNA production, processing, and degradation. To date, little is known about time‐dependent …

Many mammalian genes are transcribed during short bursts of variable frequencies and
sizes that substantially contribute to cell-to-cell variability. However, which molecular …

Recent experimental developments in genome-wide RNA quantification hold considerable
promise for systems biology. However, rigorously probing the biology of living cells requires …