Recent advancements in nucleic acid detection with microfluidic chip for molecular diagnostics

Z Li, X Xu, D Wang, X Jiang - TrAC Trends in Analytical Chemistry, 2023 - Elsevier
Abstract The coronavirus disease 2019 (COVID-19) has extensively promoted the
application of nucleic acid testing technology in the field of clinical testing. The most widely …

CRISPR/Cas Systems‐Inspired Nano/Biosensors for Detecting Infectious Viruses and Pathogenic Bacteria

X Zhang, Y Shi, G Chen, D Wu, Y Wu, G Li - Small Methods, 2022 - Wiley Online Library
Infectious pathogens cause severe human illnesses and great deaths per year worldwide.
Rapid, sensitive, and accurate detection of pathogens is of great importance for preventing …

Recent advances on CRISPR/Cas system-enabled portable detection devices for on-site agri-food safety assay

M Qiu, J Zhang, L Pang, Y Zhang, Q Zhao… - Trends in Food Science …, 2022 - Elsevier
Background Agri-food safety has been considered as one of the most important public
concerns worldwide. From farm to table, food crops and foods are extremely vulnerable to …

LAMP-based point-of-care biosensors for rapid pathogen detection

D Das, CW Lin, HS Chuang - Biosensors, 2022 - mdpi.com
Seeking optimized infectious pathogen detection tools is of primary importance to lessen the
spread of infections, allowing prompt medical attention for the infected. Among nucleic-acid …

Recent advances of food safety detection by nucleic acid isothermal amplification integrated with CRISPR/Cas

J Qiao, Z Zhao, Y Li, M Lu, S Man, S Ye… - Critical Reviews in …, 2024 - Taylor & Francis
Food safety problems have become one of the most important public health issues
worldwide. Therefore, the development of rapid, effective and robust detection is of great …

[HTML][HTML] Diagnostic techniques for rapid detection of Vibrio species

KY Loo, JWF Law, LTH Tan, P Pusparajah… - Aquaculture, 2022 - Elsevier
Background Vibrio sp. has caused several foodborne outbreaks of gastroenteritis around the
world. Given the mounting evidence that Vibrio sp. poses a significant public health threat …

A critical review of microfluidic systems for CRISPR assays

AS Avaro, JG Santiago - Lab on a Chip, 2023 - pubs.rsc.org
Reviewed are nucleic acid detection assays that incorporate clustered regularly interspaced
short palindromic repeats (CRISPR)-based diagnostics and microfluidic devices and …

Recent progress in CRISPR-based microfluidic assays and applications

P Li, H Xiong, B Yang, X Jiang, J Kong… - TrAC Trends in Analytical …, 2022 - Elsevier
In the past few years, CRISPR-based (Clustered regularly interspaced short palindromic
repeats-based) detection techniques have arisen in the field of bioanalysis, molecular …

Microfluidics: the propellant of CRISPR-based nucleic acid detection

Y Chen, S Qian, X Yu, J Wu, J Xu - Trends in Biotechnology, 2023 - cell.com
Since the discovery of collateral cleavage activity, clustered regularly interspaced short
palindromic repeats (CRISPR)/Cas systems have become the new generation of nucleic …

Temperature-programmed microfluidic CRISPR diagnostics enable rapid and automatous point-of-care testing for syphilis

B Shu, J Yang, W Chen, X Li, Y Xue, M Liu, X Yin… - Chemical Engineering …, 2024 - Elsevier
The rising incidence of syphilis highlights the urgent need to develop molecular tests of
Treponema pallidum at the point-of-care (POC). Traditional molecular approaches based on …