Light sheet fluorescence microscopy

EHK Stelzer, F Strobl, BJ Chang, F Preusser… - Nature Reviews …, 2021 - nature.com
Light sheet fluorescence microscopy (LSFM) uses a thin sheet of light to excite only
fluorophores within the focal volume. Light sheet microscopes (LSMs) have a true optical …

A survey of physical methods for studying nuclear mechanics and mechanobiology

CM Hobson, MR Falvo, R Superfine - APL bioengineering, 2021 - pubs.aip.org
It is increasingly appreciated that the cell nucleus is not only a home for DNA but also a
complex material that resists physical deformations and dynamically responds to external …

Protein motion in the nucleus: from anomalous diffusion to weak interactions

M Woringer, X Darzacq - Biochemical Society Transactions, 2018 - portlandpress.com
Understanding how transcription factors (TFs) regulate mammalian gene expression in
space and time is a central topic in biology. To activate a gene, a TF has first to diffuse in the …

Single-particle tracking reveals anti-persistent subdiffusion in cell extracts

K Speckner, M Weiss - Entropy, 2021 - mdpi.com
Single-particle tracking (SPT) has become a powerful tool to quantify transport phenomena
in complex media with unprecedented detail. Based on the reconstruction of individual …

Measuring mobility in chromatin by intensity-sorted FCS

M Di Bona, MA Mancini, D Mazza, G Vicidomini… - Biophysical …, 2019 - cell.com
The architectural organization of chromatin can play an important role in genome regulation
by affecting the mobility of molecules within its surroundings via binding interactions and …

Functional fluorescence microscopy imaging: quantitative scanning-free confocal fluorescence microscopy for the characterization of fast dynamic processes in live …

AJ Krmpot, SN Nikolic, S Oasa… - Analytical …, 2019 - ACS Publications
Functional fluorescence microscopy imaging (fFMI), a time-resolved (21 μs/frame) confocal
fluorescence microscopy imaging technique without scanning, is developed for quantitative …

Local raster image correlation spectroscopy generates high-resolution intracellular diffusion maps

L Scipioni, M Di Bona, G Vicidomini, A Diaspro… - Communications …, 2018 - nature.com
Raster image correlation spectroscopy (RICS) is a powerful method for measuring molecular
diffusion in live cells directly from images acquired on a laser scanning microscope …

Dynamic cellular cartography: mapping the local determinants of oligodendrocyte transcription factor 2 (OLIG2) function in live cells using massively parallel …

S Oasa, AJ Krmpot, SN Nikolic, AHA Clayton… - Analytical …, 2021 - ACS Publications
Compartmentalization and integration of molecular processes through diffusion are basic
mechanisms through which cells perform biological functions. To characterize these …

Spatially resolving size effects on diffusivity in nanoporous extracellular matrix-like materials with fluorescence correlation spectroscopy super-resolution optical …

S Chatterjee, SN Kramer, B Wellnitz… - The Journal of …, 2023 - ACS Publications
It is well documented that the nanoscale structures within porous microenvironments greatly
impact the diffusion dynamics of molecules. However, how the interaction between the …

Application of genetically encoded photoconvertible protein SAASoti for the study of enzyme activity in a single live cell by fluorescence correlation microscopy

ID Solovyev, LG Maloshenok, AP Savitsky - Materials, 2022 - mdpi.com
Fluorescent Correlation Spectroscopy (FCS) allows us to determine interactions of labeled
proteins or changes in the oligomeric state. The FCS method needs a low amount of …