Recent achievements obtained by chloroplast transformation

M Adem, D Beyene, T Feyissa - Plant Methods, 2017 - Springer
Chloroplasts play a great role for sustained wellbeing of life on the planet. They have the
power and raw materials that can be used as sophisticated biological factories. They are rich …

Mycobacterium tuberculosis Shikimate Pathway Enzymes as Targets for the Rational Design of Anti-Tuberculosis Drugs

JES Nunes, MA Duque, TF de Freitas, L Galina… - Molecules, 2020 - mdpi.com
Roughly a third of the world's population is estimated to have latent Mycobacterium
tuberculosis infection, being at risk of developing active tuberculosis (TB) during their …

Catalytic and structural effects of flexible loop deletion in organophosphorus hydrolase enzyme: A thermostability improvement mechanism

G Farnoosh, K Khajeh, M Mohammadi… - Journal of …, 2020 - Springer
Thermostability improvement of enzymes used industrially or commercially would develop
their capacity and commercial potential due to increased enzymatic competence and cost …

Characterization of a Glyphosate-Tolerant Enzyme from Streptomyces svecius: A Distinct Class of 5-Enolpyruvylshikimate-3-phosphate Synthases

SL Griffin, JR Chekan, JM Lira… - Journal of Agricultural …, 2021 - ACS Publications
Natural and modified versions of the 5-enolpyruvylshikimate-3-phosphate synthase (epsps)
gene have been used to confer tolerance to the broad-spectrum herbicide glyphosate in a …

Plant plastid engineering

SH Wani, N Haider, H Kumar, NB Singh - Current genomics, 2010 - ingentaconnect.com
Genetic material in plants is distributed into nucleus, plastids and mitochondria. Plastid has
a central role of carrying out photosynthesis in plant cells. Plastid transformation is becoming …

Engineering and introduction of de novo disulphide bridges in organophosphorus hydrolase enzyme for thermostability improvement

G Farnoosh, K Khajeh, AM Latifi, H Aghamollaei - Journal of biosciences, 2016 - Springer
The organophosphorus hydrolase (OPH) has been used to degrade organophosphorus
chemicals, as one of the most frequently used decontamination methods. Under chemical …

[PDF][PDF] Regeneration of glyphosate-tolerant Nicotiana tabacum after plastid transformation with a mutated variant of bacterial aroA gene

RM FATHI, AH Salmanian, A Mousavi, SH HASHEMI… - 2009 - sid.ir
Presence of antibiotic resistance markers has always been considered as one of the main
safety concerns in transgenic plants and their derived products. Elimination of antibiotic …

EPSP synthase flexibility is determinant to its function: computational molecular dynamics and metadynamics studies

LFSM Timmers, AMS Neto, RW Montalvão… - Journal of molecular …, 2017 - Springer
Flexibility is involved in a wide range of biological processes, such as protein assembly and
binding recognition. EPSP synthase is an enzyme that must undergo a large conformational …

Over-expression of the Gr5 aroA gene from glyphosate-contaminated soil confers high tolerance to glyphosate in tobacco

J Wang, K Zuo, W Lu, Y Zhu, C Ye, M Lin, K Tang - Molecular breeding, 2014 - Springer
Herbicide resistance is the most widely used transgenic crop trait for broad-spectrum control
of weeds. Here we report a novel 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) …

Evidence regarding the hypothesis that the histidine–histidine contact pairs may affect protein stability

K Haghani, K Khajeh, H Naderi-Manesh… - International journal of …, 2012 - Elsevier
It has been lately proposed that the interaction between like-charged residues stabilizes the
native state of proteins. To explore this, we created a histidine–histidine pair in the Ca-III …