Single-cell RNA-seq methods to interrogate virus-host interactions
The twenty-first century has seen the emergence of many epidemic and pandemic viruses,
with the most recent being the SARS-CoV-2-driven COVID-19 pandemic. As obligate …
with the most recent being the SARS-CoV-2-driven COVID-19 pandemic. As obligate …
Dissecting human population variation in single-cell responses to SARS-CoV-2
Humans display substantial interindividual clinical variability after SARS-CoV-2 infection,–,
the genetic and immunological basis of which has begun to be deciphered. However, the …
the genetic and immunological basis of which has begun to be deciphered. However, the …
Virus-like particles: Measures and biological functions
Virus-like particles resemble infectious virus particles in size, shape, and molecular
composition; however, they fail to productively infect host cells. Historically, the presence of …
composition; however, they fail to productively infect host cells. Historically, the presence of …
Segment-specific kinetics of mRNA, cRNA, and vRNA accumulation during influenza virus infection
Influenza A virus (IAV) is a segmented negative-sense RNA virus and is the cause of major
epidemics and pandemics. The replication of IAV is complex, involving the production of …
epidemics and pandemics. The replication of IAV is complex, involving the production of …
Single-cell herpes simplex virus type 1 infection of neurons using drop-based microfluidics reveals heterogeneous replication kinetics
Single-cell analyses of viral infections reveal heterogeneity that is not detected by traditional
population-level studies. This study applies drop-based microfluidics to investigate the …
population-level studies. This study applies drop-based microfluidics to investigate the …
High-throughput droplet-based analysis of influenza A virus genetic reassortment by single-virus RNA sequencing
KY Chen, J Karuppusamy, MB O'Neill… - Proceedings of the …, 2023 - National Acad Sciences
The segmented RNA genome of influenza A viruses (IAVs) enables viral evolution through
genetic reassortment after multiple IAVs coinfect the same cell, leading to viruses harboring …
genetic reassortment after multiple IAVs coinfect the same cell, leading to viruses harboring …
Influenza virus transcription and progeny production are poorly correlated in single cells
The ultimate success of a viral infection at the cellular level is determined by the number of
progeny virions produced. However, most single-cell studies of infection quantify the …
progeny virions produced. However, most single-cell studies of infection quantify the …
Single‐cell transcriptomics reveals immune response of intestinal cell types to viral infection
S Triana, ML Stanifer, C Metz‐Zumaran… - Molecular systems …, 2021 - embopress.org
Human intestinal epithelial cells form a primary barrier protecting us from pathogens, yet
only limited knowledge is available about individual contribution of each cell type to …
only limited knowledge is available about individual contribution of each cell type to …
Single-cell and bulk RNA-sequencing reveal differences in monocyte susceptibility to influenza A virus infection between Africans and Europeans
MB O'neill, H Quach, J Pothlichet, Y Aquino… - Frontiers in …, 2021 - frontiersin.org
There is considerable inter-individual and inter-population variability in response to viruses.
The potential of monocytes to elicit type-I interferon responses has attracted attention to their …
The potential of monocytes to elicit type-I interferon responses has attracted attention to their …
Droplet microfluidics for high-resolution virology
W Jing, HS Han - 2022 - ACS Publications
Microfluidics has enabled a new era of cellular and molecular assays due to the small length
scales, parallelization, and the modularity of various analysis and actuation functions …
scales, parallelization, and the modularity of various analysis and actuation functions …