Although bacteria frequently live as unicellular organisms, many spend at least part of their
lives in complex communities, and some have adopted truly multicellular lifestyles and have …

Bacteria display an abundance of cellular forms and can change shape during their life
cycle. Many plausible models regarding the functional significance of cell morphology have …

Bacteria frequently exchange metabolites by diffusion through the extracellular environment,
yet it remains generally unclear whether bacteria can also use cell–cell connections to …

Cryo-electron tomography (CET) produces three-dimensional images of cells in a near-
native state at macromolecular resolution, but identifying structures of interest can be …

Molecular self-organziation, also regarded as pattern formation, is crucial for the correct
distribution of cellular content. The processes leading to spatiotemporal patterns often …

Electron cryotomography (ECT) provides three-dimensional views of macromolecular
complexes inside cells in a native frozen–hydrated state. Over the last two decades, ECT …

First isolated and classified in the 1960s, Caulobacter crescentus has been instrumental in
the study of bacterial cell biology and differentiation. C. crescentus is a Gram-negative …

Biological processes are inherently multi-scale, and supramolecular complexes at the
nanoscale determine changes at the cellular scale and beyond. Single-molecule …

Electron cryotomography (ECT) enables intact cells to be visualized in 3D in an essentially
native state to'macromolecular'(∼ 4 nm) resolution, revealing the basic architectures of …

Studying bacterial cell envelope architecture with electron microscopy is challenging due to
the poor preservation of microbial ultrastructure with traditional methods. Here, we …