Protein stability is a topic of major interest for the biotechnology, pharmaceutical and food
industries, in addition to being a daily consideration for academic researchers studying …

Escherichia coli has long been the primary prokaryotic host for the synthesis of heterologous
proteins. Recent advances have been made in the expression of complex proteins as …

APEX is an engineered peroxidase that functions as an electron microscopy tag and a
promiscuous labeling enzyme for live-cell proteomics. Because limited sensitivity precludes …

Soy leghemoglobin is a key food additive that imparts meaty flavor and color to meat
analogs. Here, a Pichia pastoris strain capable of high-yield secretory production of …

MicroRNAs (miRNAs) regulate the expression of a large number of protein-coding genes.
Their primary transcripts (pri-miRNAs) have to undergo multiple processing steps to reach …

Human cystathionine β-synthase catalyzes the first step in the catabolic removal of the toxic
metabolite, homocysteine. It is unique in being dependent on both pyridoxal phosphate …

Cytochromes c (cyt c) and c 1 are heme proteins that are essential for aerobic respiration.
Release of cyt c from mitochondria is an important signal in apoptosis initiation. Biogenesis …

In mammals, cystathionine β-synthase catalyzes the first step in the transsulfuration pathway
which provides an avenue for the conversion of the essential amino acid, methionine, to …

The catabolism of heme, generating biliverdin, carbon monoxide, and free iron, is mediated
by heme oxygenase (HO). One form of this of this enzyme, heme oxygenase-1, is inducible …

Sulfur-containing amino acids play an important role in a variety of cellular functions such as
protein synthesis, methylation, and polyamine and glutathione synthesis. We cloned and …