Analysis of transcriptome and epitranscriptome in plants using PacBio Iso-Seq and nanopore-based direct RNA sequencing
L Zhao, H Zhang, MV Kohnen, KVSK Prasad… - Frontiers in …, 2019 - frontiersin.org
Nanopore sequencing from Oxford Nanopore Technologies (ONT) and Pacific BioSciences
(PacBio) single-molecule real-time (SMRT) long-read isoform sequencing (Iso-Seq) are …
(PacBio) single-molecule real-time (SMRT) long-read isoform sequencing (Iso-Seq) are …
RNA sequencing and its applications in cancer and rare diseases
S Ergin, N Kherad, M Alagoz - Molecular Biology Reports, 2022 - Springer
With the invention of RNA sequencing over a decade ago, diagnosis and identification of the
gene-related diseases entered a new phase that enabled more accurate analysis of the …
gene-related diseases entered a new phase that enabled more accurate analysis of the …
Direct RNA nanopore sequencing of full-length coronavirus genomes provides novel insights into structural variants and enables modification analysis
Sequence analyses of RNA virus genomes remain challenging owing to the exceptional
genetic plasticity of these viruses. Because of high mutation and recombination rates …
genetic plasticity of these viruses. Because of high mutation and recombination rates …
A comparative evaluation of hybrid error correction methods for error-prone long reads
S Fu, A Wang, KF Au - Genome biology, 2019 - Springer
Background Third-generation sequencing technologies have advanced the progress of the
biological research by generating reads that are substantially longer than second …
biological research by generating reads that are substantially longer than second …
Integrative functional genomics decodes herpes simplex virus 1
The predicted 80 open reading frames (ORFs) of herpes simplex virus 1 (HSV-1) have been
intensively studied for decades. Here, we unravel the complete viral transcriptome and …
intensively studied for decades. Here, we unravel the complete viral transcriptome and …
The antiviral activity of kaempferol against pseudorabies virus in mice
L Li, R Wang, H Hu, X Chen, Z Yin, X Liang, C He… - BMC veterinary …, 2021 - Springer
Background Pseudorabies virus (PRV), a member of the Alphaherpesviruses, is one of the
most important pathogens that harm the global pig industry. Accumulated evidence …
most important pathogens that harm the global pig industry. Accumulated evidence …
Long-read sequencing–a powerful tool in viral transcriptome research
Long-read sequencing (LRS) has become increasingly popular due to its strengths in de
novo assembly and in resolving complex DNA regions as well as in determining full-length …
novo assembly and in resolving complex DNA regions as well as in determining full-length …
Long-read sequencing uncovers a complex transcriptome topology in varicella zoster virus
Background Varicella zoster virus (VZV) is a human pathogenic alphaherpesvirus harboring
a relatively large DNA molecule. The VZV transcriptome has already been analyzed by …
a relatively large DNA molecule. The VZV transcriptome has already been analyzed by …
Dynamic nanopore long-read sequencing analysis of HIV-1 splicing events during the early steps of infection
N Nguyen Quang, S Goudey, E Ségéral, A Mohammad… - Retrovirology, 2020 - Springer
Background Alternative splicing is a key step in Human Immunodeficiency Virus type 1 (HIV-
1) replication that is tightly regulated both temporally and spatially. More than 50 different …
1) replication that is tightly regulated both temporally and spatially. More than 50 different …
Identification of herpesvirus transcripts from genomic regions around the replication origins
Long-read sequencing (LRS) techniques enable the identification of full-length RNA
molecules in a single run eliminating the need for additional assembly steps. LRS research …
molecules in a single run eliminating the need for additional assembly steps. LRS research …