Genetic alphabet expansion technology by creating unnatural base pairs
Recent advancements in the creation of artificial extra base pairs (unnatural base pairs,
UBPs) are opening the door to a new research area, xenobiology, and genetic alphabet …
UBPs) are opening the door to a new research area, xenobiology, and genetic alphabet …
High-affinity DNA aptamer generation targeting von Willebrand factor A1-domain by genetic alphabet expansion for systematic evolution of ligands by exponential …
The novel evolutionary engineering method ExSELEX (genetic alphabet expansion for
systematic evolution of ligands by exponential enrichment) provides high-affinity DNA …
systematic evolution of ligands by exponential enrichment) provides high-affinity DNA …
Nucleoside triphosphates—building blocks for the modification of nucleic acids
M Hollenstein - Molecules, 2012 - mdpi.com
Nucleoside triphosphates are moldable entities that can easily be functionalized at various
locations. The enzymatic polymerization of these modified triphosphate analogues …
locations. The enzymatic polymerization of these modified triphosphate analogues …
Reversible bond formation enables the replication and amplification of a crosslinking salen complex as an orthogonal base pair
C Kaul, M Müller, M Wagner, S Schneider, T Carell - Nature chemistry, 2011 - nature.com
The universal genetic code relies on two hydrogen-bonded Watson–Crick base pairs that
can form 64 triplet codons. This places a limit on the number of amino acids that can be …
can form 64 triplet codons. This places a limit on the number of amino acids that can be …
Fluorescent nucleoside analogs: probes for investigating nucleic acid structure and function
AA Tanpure, MG Pawar… - Israel Journal of …, 2013 - Wiley Online Library
Base‐modified fluorescent nucleoside analog probes have been very valuable in the study
of nucleic acid structure and function. Many of them structurally resemble natural bases, and …
of nucleic acid structure and function. Many of them structurally resemble natural bases, and …
Structural basis of transcription recognition of a hydrophobic unnatural base pair by T7 RNA polymerase
Abstract Bacteriophage T7 RNA polymerase (T7 RNAP) is widely used for synthesizing RNA
molecules with synthetic modifications and unnatural base pairs (UBPs) for a variety of …
molecules with synthetic modifications and unnatural base pairs (UBPs) for a variety of …
Creation of unnatural base pairs for genetic alphabet expansion toward synthetic xenobiology
Highlights•A series of unnatural base pairs that function as a third base pair in the central
dogma were developed.•High-affinity DNA aptamers that specifically bind to target proteins …
dogma were developed.•High-affinity DNA aptamers that specifically bind to target proteins …
Unnatural base pair systems toward the expansion of the genetic alphabet in the central dogma
Toward the expansion of the genetic alphabet of DNA, several artificial third base pairs
(unnatural base pairs) have been created. Synthetic DNAs containing the unnatural base …
(unnatural base pairs) have been created. Synthetic DNAs containing the unnatural base …
Alternative DNA base-pairs: from efforts to expand the genetic code to potential material applications
F Wojciechowski, CJ Leumann - Chemical Society Reviews, 2011 - pubs.rsc.org
The complementary Watson–Crick base-pairs, A: T and G: C, have long been recognized as
pivotal to both the stability of the DNA double helix and replication/transcription. Recently …
pivotal to both the stability of the DNA double helix and replication/transcription. Recently …