Development and use of molecular markers: past and present
A Grover, PC Sharma - Critical reviews in biotechnology, 2016 - Taylor & Francis
Molecular markers, due to their stability, cost-effectiveness and ease of use provide an
immensely popular tool for a variety of applications including genome mapping, gene …
immensely popular tool for a variety of applications including genome mapping, gene …
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
RK Saiki, DH Gelfand, S Stoffel, SJ Scharf, R Higuchi… - Science, 1988 - science.org
A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the
polymerase chain reaction. The enzyme, isolated from Thermus aquaticus, greatly simplifies …
polymerase chain reaction. The enzyme, isolated from Thermus aquaticus, greatly simplifies …
Adaptive protein evolution at the Adh locus in Drosophila
JH McDonald, M Kreitman - Nature, 1991 - nature.com
PROTEINS often differ in amino-acid sequence across species. This difference has evolved
by the accumulation of neutral mutations by random drift, the fixation of adaptive mutations …
by the accumulation of neutral mutations by random drift, the fixation of adaptive mutations …
The shared epitope hypothesis. An approach to understanding the molecular genetics of susceptibility to rheumatoid arthritis
PK Gregersen, J Silver, RJ Winchester - Arthritis & …, 1987 - api.taylorfrancis.com
Much work investigating the inheritance of RA has focussed on the associations between
HLA class II serological specificities and disease. This has demonstrated association with …
HLA class II serological specificities and disease. This has demonstrated association with …
Digital pcr
B Vogelstein, KW Kinzler - Proceedings of the National …, 1999 - National Acad Sciences
The identification of predefined mutations expected to be present in a minor fraction of a cell
population is important for a variety of basic research and clinical applications. Here, we …
population is important for a variety of basic research and clinical applications. Here, we …
Simultaneous amplification and detection of specific DNA sequences
R Higuchi, G Dollinger, PS Walsh, R Griffith - Bio/technology, 1992 - nature.com
We have enhanced the polymerase chain reaction (PCR) such that specific DNA sequences
can be detected without opening the reaction tube. This enhancement requires the addition …
can be detected without opening the reaction tube. This enhancement requires the addition …
Deletion screening of the Duchenne muscular dystrophy locus via multiplex DNA amplification
JS Chamberlain, RA Gibbs, JE Rainer… - Nucleic acids …, 1988 - academic.oup.com
The application of recombinant DNA technology to prenatal diagnosis of many recessively
inherited X-linked diseases is complicated by a high frequency of heterogenous, new …
inherited X-linked diseases is complicated by a high frequency of heterogenous, new …
Mitochondrial ribosomal RNA mutation associated with both antibiotic–induced and non–syndromic deafness
TR Prezant, JV Agapian, MC Bohlman, X Bu, S Öztas… - Nature …, 1993 - nature.com
Maternally transmitted non–syndromic deafness was described recently both in pedigrees
with susceptibility to aminoglycoside ototoxicity and in a large Arab–Israeli pedigree …
with susceptibility to aminoglycoside ototoxicity and in a large Arab–Israeli pedigree …
HLA‐DR typing by PCR amplification with sequence‐specific primers (PCR‐SSP) in 2 hours: an alternative to serological DR typing in clinical practice including donor …
O Olerup, H Zetterquist - Tissue antigens, 1992 - Wiley Online Library
In most PCR‐based tissue typing techniques the PCR amplification is followed by a post‐
amplification specificity step. In typing by PCR amplification with sequence‐specific primers …
amplification specificity step. In typing by PCR amplification with sequence‐specific primers …