Techniques for the analysis of protein-protein interactions in vivo

S Xing, N Wallmeroth, KW Berendzen… - Plant physiology, 2016 - academic.oup.com
Identifying key players and their interactions is fundamental for understanding biochemical
mechanisms at the molecular level. The ever-increasing number of alternative ways to …

[HTML][HTML] Polishing the craft of genetic diversity creation in directed evolution

KL Tee, TS Wong - Biotechnology advances, 2013 - Elsevier
Genetic diversity creation is a core technology in directed evolution where a high quality
mutant library is crucial to its success. Owing to its importance, the technology in genetic …

Structure of the homodimeric androgen receptor ligand-binding domain

M Nadal, S Prekovic, N Gallastegui, C Helsen… - Nature …, 2017 - nature.com
The androgen receptor (AR) plays a crucial role in normal physiology, development and
metabolism as well as in the aetiology and treatment of diverse pathologies such as …

The Arabidopsis R-SNARE VAMP721 Interacts with KAT1 and KC1 K+ Channels to Moderate K+ Current at the Plasma Membrane

B Zhang, R Karnik, Y Wang, N Wallmeroth… - The Plant …, 2015 - academic.oup.com
SNARE (soluble N-ethylmaleimide-sensitive factor protein attachment protein receptor)
proteins drive vesicle traffic, delivering membrane and cargo to target sites within the cell …

Binary 2in1 vectors improve in planta (co) localization and dynamic protein interaction studies

A Hecker, N Wallmeroth, S Peter, MR Blatt… - Plant …, 2015 - academic.oup.com
Fluorescence-based protein-protein interaction techniques are vital tools for understanding
in vivo cellular functions on a mechanistic level. However, only under the condition of highly …

[PDF][PDF] Barriers to genetic manipulation of Enterococci: Current approaches and future directions

AL Krause, TP Stinear, IR Monk - FEMS Microbiology Reviews, 2022 - academic.oup.com
Enterococcus faecalis and Enterococcus faecium are Gram-positive commensal gut bacteria
that can also cause fatal infections. To study clinically relevant multi-drug resistant E …

Loss of GET pathway orthologs in Arabidopsis thaliana causes root hair growth defects and affects SNARE abundance

S Xing, DG Mehlhorn, N Wallmeroth… - Proceedings of the …, 2017 - National Acad Sciences
Soluble N-ethylmaleimide–sensitive factor attachment protein receptor (SNARE) proteins
are key players in cellular trafficking and coordinate vital cellular processes, such as …

TOR signaling regulates liquid phase separation of the SMN complex governing snRNP biogenesis

M Schilling, AB Prusty, B Boysen, FS Oppermann… - Cell reports, 2021 - cell.com
The activity of the SMN complex in promoting the assembly of pre-mRNA processing
UsnRNPs correlates with condensation of the complex in nuclear Cajal bodies. While …

A vesicle-trafficking protein commandeers Kv channel voltage sensors for voltage-dependent secretion

C Grefen, R Karnik, E Larson, C Lefoulon, Y Wang… - Nature plants, 2015 - nature.com
Growth in plants depends on ion transport for osmotic solute uptake and secretory
membrane trafficking to deliver material for wall remodelling and cell expansion. The …

Binding of SEC11 indicates its role in SNARE recycling after vesicle fusion and identifies two pathways for vesicular traffic to the plasma membrane

R Karnik, B Zhang, S Waghmare, C Aderhold… - The Plant …, 2015 - academic.oup.com
SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) proteins
drive vesicle fusion in all eukaryotes and contribute to homeostasis, pathogen defense, cell …