Highlights•PCR products can be used to make proteins quickly and easily in a cell-free
system.•Recent advances have widened the application space of such cell-free …

The engineering of and mastery over biological parts has catalyzed the emergence of
synthetic biology. This field has grown exponentially in the past decade. As increasingly …

Cell-free protein synthesis (CFPS) is a versatile tool for rapid recombinant protein production
and engineering. One drawback of cell-free technology is the necessity to store the major …

Incorporation of noncanonical amino acids (ncAAs) into proteins opens new opportunities in
biotechnology and synthetic biology. Pyrrolysine (Pyl)‐based ncAAs are some of the most …

Highlights•CFPS systems allow for highly optimizable and rapid protein production.•CFPS
overcomes limitations in current vaccine production and expands vaccine …

Highlights•Protein containing unnatural amino acid is produced using alternate energy
systems.•PCR linear DNA template expression of proteins containing unnatural amino …

The effectiveness and economics of polyvinyl sulfonic acid (PVSA) as a ribonuclease
inhibitor for in vitro systems is reported. PVSA was shown to inhibit RNA cleavage in the …

Escherichia coli-based cell-free protein synthesis is a powerful emerging tool for protein
engineering due to the open, accessible nature of the reaction and its straightforward …

Emancipating sense codons toward a minimized genetic code is of significant interest to
science and engineering. A key approach toward sense codon emancipation is the targeted …

The interdisciplinary nature of synthetic biology merges engineering principles with biology
and provides innovative solutions for issues in the biomanufacturing industry. To develop …