Fluorescence lifetime imaging microscopy: fundamentals and advances in instrumentation, analysis, and applications
Significance: Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique to
distinguish the unique molecular environment of fluorophores. FLIM measures the time a …
distinguish the unique molecular environment of fluorophores. FLIM measures the time a …
Fluorescence lifetime measurements and biological imaging
MY Berezin, S Achilefu - Chemical reviews, 2010 - ACS Publications
When a molecule absorbs a photon of appropriate energy, a chain of photophysical events
ensues, such as internal conversion or vibrational relaxation (loss of energy in the absence …
ensues, such as internal conversion or vibrational relaxation (loss of energy in the absence …
Fluorescent proteins and their applications in imaging living cells and tissues
Green fluorescent protein (GFP) from the jellyfish Aequorea victoria and its homologs from
diverse marine animals are widely used as universal genetically encoded fluorescent labels …
diverse marine animals are widely used as universal genetically encoded fluorescent labels …
CYFIP1 coordinates mRNA translation and cytoskeleton remodeling to ensure proper dendritic spine formation
S De Rubeis, E Pasciuto, KW Li, E Fernández… - Neuron, 2013 - cell.com
The CYFIP1/SRA1 gene is located in a chromosomal region linked to various neurological
disorders, including intellectual disability, autism, and schizophrenia. CYFIP1 plays a dual …
disorders, including intellectual disability, autism, and schizophrenia. CYFIP1 plays a dual …
Single cell optical imaging and spectroscopy
AS Stender, K Marchuk, C Liu, S Sander… - Chemical …, 2013 - ACS Publications
In his 1665 treatise, Micrographia, Robert Hooke described the many observations he had
made using a microscope, including compartment-like structures in cork samples that he …
made using a microscope, including compartment-like structures in cork samples that he …
Stem cell signaling in Arabidopsis requires CRN to localize CLV2 to the plasma membrane
A Bleckmann, S Weidtkamp-Peters… - Plant …, 2010 - academic.oup.com
Stem cell number in shoot and floral meristems of Arabidopsis (Arabidopsis thaliana) is
regulated by the CLAVATA3 (CLV3) signaling pathway. Perception of the CLV3 peptide …
regulated by the CLAVATA3 (CLV3) signaling pathway. Perception of the CLV3 peptide …
Persister Escherichia coli cells have a lower intracellular pH than susceptible cells but maintain their pH in response to antibiotic treatment
Persister and viable but non-culturable (VBNC) cells are two clonal subpopulations that can
survive multidrug exposure via a plethora of putative molecular mechanisms. Here, we …
survive multidrug exposure via a plethora of putative molecular mechanisms. Here, we …
Mitochondrial BCL‐2 inhibits AMBRA1‐induced autophagy
F Strappazzon, M Vietri‐Rudan, S Campello… - The EMBO …, 2011 - embopress.org
BECLIN 1 is a central player in macroautophagy. AMBRA1, a BECLIN 1‐interacting protein,
positively regulates the BECLIN 1‐dependent programme of autophagy. In this study, we …
positively regulates the BECLIN 1‐dependent programme of autophagy. In this study, we …
Fluorescence resonance energy transfer microscopy as demonstrated by measuring the activation of the serine/threonine kinase Akt
This protocol describes procedures for performing fluorescence resonance energy transfer
(FRET) microscopy analysis by three different methods: acceptor photobleaching, sensitized …
(FRET) microscopy analysis by three different methods: acceptor photobleaching, sensitized …
EIN2, the central regulator of ethylene signalling, is localized at the ER membrane where it interacts with the ethylene receptor ETR1
MMA Bisson, A Bleckmann, S Allekotte… - Biochemical …, 2009 - portlandpress.com
Genetic studies have identified the membrane protein EIN2 (ethylene insensitive 2) as a
central component of ethylene signalling in Arabidopsis. In addition, EIN2 might take part in …
central component of ethylene signalling in Arabidopsis. In addition, EIN2 might take part in …