Induced pluripotent stem cell technology: a decade of progress

Y Shi, H Inoue, JC Wu, S Yamanaka - Nature reviews Drug discovery, 2017 - nature.com
Since the advent of induced pluripotent stem cell (iPSC) technology a decade ago,
enormous progress has been made in stem cell biology and regenerative medicine. Human …

Aging in a dish: iPSC-derived and directly induced neurons for studying brain aging and age-related neurodegenerative diseases

J Mertens, D Reid, S Lau, Y Kim… - Annual review of …, 2018 - annualreviews.org
Age-associated neurological diseases represent a profound challenge in biomedical
research as we are still struggling to understand the interface between the aging process …

Age-dependent instability of mature neuronal fate in induced neurons from Alzheimer's patients

J Mertens, JR Herdy, L Traxler, ST Schafer… - Cell stem cell, 2021 - cell.com
Sporadic Alzheimer's disease (AD) exclusively affects elderly people. Using direct
conversion of AD patient fibroblasts into induced neurons (iNs), we generated an age …

Directly reprogrammed human neurons retain aging-associated transcriptomic signatures and reveal age-related nucleocytoplasmic defects

J Mertens, ACM Paquola, M Ku, E Hatch, L Böhnke… - Cell stem cell, 2015 - cell.com
Aging is a major risk factor for many human diseases, and in vitro generation of human
neurons is an attractive approach for modeling aging-related brain disorders. However …

Alzheimer's disease: treatment strategies and their limitations

E Passeri, K Elkhoury, M Morsink, K Broersen… - International journal of …, 2022 - mdpi.com
Alzheimer's disease (AD) is the most frequent case of neurodegenerative disease and is
becoming a major public health problem all over the world. Many therapeutic strategies …

[HTML][HTML] iPSC-based compound screening and in vitro trials identify a synergistic anti-amyloid β combination for Alzheimer's disease

T Kondo, K Imamura, M Funayama, K Tsukita… - Cell reports, 2017 - cell.com
In the process of drug development, in vitro studies do not always adequately predict human-
specific drug responsiveness in clinical trials. Here, we applied the advantage of human …

Investigating human disease using stem cell models

JL Sterneckert, P Reinhardt, HR Schöler - Nature Reviews Genetics, 2014 - nature.com
Tractable and accurate disease models are essential for understanding disease
pathogenesis and for developing new therapeutics. As stem cells are capable of self …

Analysis of short tandem repeat expansions and their methylation state with nanopore sequencing

P Giesselmann, B Brändl, E Raimondeau… - Nature …, 2019 - nature.com
Expansions of short tandem repeats are genetic variants that have been implicated in
several neuropsychiatric and other disorders, but their assessment remains challenging with …

iPS cell technologies: significance and applications to CNS regeneration and disease

H Okano, S Yamanaka - Molecular brain, 2014 - Springer
In 2006, we demonstrated that mature somatic cells can be reprogrammed to a pluripotent
state by gene transfer, generating induced pluripotent stem (iPS) cells. Since that time, there …

[HTML][HTML] Oxidative stress and altered mitochondrial protein expression in the absence of amyloid-β and tau pathology in iPSC-derived neurons from sporadic …

JH Birnbaum, D Wanner, AF Gietl, A Saake… - Stem cell …, 2018 - Elsevier
Mitochondrial dysfunction is a prominent feature of Alzheimer's disease (AD) and increased
production of reactive oxygen species (ROS) has been described in postmortem brain …