Retinitis Pigmentosa (RP) is a hereditary retinopathy that affects about 2.5 million people
worldwide. It is characterized with progressive loss of rods and cones and causes severe …

RNA-guided Cas9 nucleases derived from clustered regularly interspaced short palindromic
repeats (CRISPR)-Cas systems have dramatically transformed our ability to edit the …

The low photostability of fluorescent proteins is a limiting factor in many applications of
fluorescence microscopy. Here we present StayGold, a green fluorescent protein (GFP) …

Vectors derived from human immunodeficiency virus (HIV) are highly efficient vehicles for in
vivo gene delivery. However, their biosafety is of major concern. Here we exploit the …

The cell-cycle transition from G 1 to S phase has been difficult to visualize. We have
harnessed antiphase oscillating proteins that mark cell-cycle transitions in order to develop …

The concept behind gene therapy is simple—by delivering corrective genetic material to the
cells of a patient the symptoms of disease can be alleviated. But seven years after the first …

We have constructed a new series of lentivirus vectors based on human immunodeficiency
virus type 1 (HIV-1) that can transduce nondividing cells. The U3 region of the 5′ long …

The large number of candidate genes made available by comprehensive genome analysis
requires that relatively rapid techniques for the study of function be developed. Here, we …

Background: The amplification of oncogenes in cancer cells is often mediated by paired
acentric chromatin bodies called double minute chromosomes (DMs), which can accumulate …

In adult hippocampus, new neurons are continuously generated from neural stem cells
(NSCs), but the molecular mechanisms regulating adult neurogenesis remain elusive. We …