Next-generation sequencing for the detection of microbial agents in avian clinical samples

CL Afonso, AM Afonso - Veterinary Sciences, 2023 - mdpi.com
Simple Summary Significant progress in next-generation sequencing (NGS) is positioning
this technology as a key tool to be utilized in clinical diagnosis of disease agents and/or for …

Rapid genotyping of porcine reproductive and respiratory syndrome virus (PRRSV) using MinION nanopore sequencing

LC Caserta, J Zhang, P Piñeyro, DG Diel - Plos one, 2023 - journals.plos.org
The global distribution and constant evolution are challenges for the control of porcine
reproductive and respiratory syndrome virus (PRRSV), one of the most important viruses …

Randomly primed, strand-switching, MinION-based sequencing for the detection and characterization of cultured RNA viruses

KT Young, KK Lahmers, HS Sellers… - Journal of …, 2021 - journals.sagepub.com
RNA viruses rapidly mutate, which can result in increased virulence, increased escape from
vaccine protection, and false-negative detection results. Targeted detection methods have a …

Comparable outcomes from long and short read random sequencing of total RNA for detection of pathogens in chicken respiratory samples

SL Butt, HM Kariithi, JD Volkening, TL Taylor… - Frontiers in Veterinary …, 2022 - frontiersin.org
Co-infections of avian species with different RNA viruses and pathogenic bacteria are often
misdiagnosed or incompletely characterized using targeted diagnostic methods, which …

An Amplicon-Based Application for the Whole-Genome Sequencing of GI-19 Lineage Infectious Bronchitis Virus Directly from Clinical Samples

HD Le, TN Thai, JK Kim, HS Song, M Her, XT Tran… - Viruses, 2024 - mdpi.com
Infectious bronchitis virus (IBV) causes a highly contagious respiratory disease in chickens,
leading to significant economic losses in the poultry industry worldwide. IBV exhibits a high …

The efficacy of the prime-boost regimen for heterologous infectious bronchitis vaccines mandates the administration of homologous vaccines

AAG Al-Kubati, MG Hemida, AIA Al-Mubarak - VirusDisease, 2022 - Springer
Infectious bronchitis virus (IBV) has been frequently reported in chickens worldwide,
including in the Eastern Region of Saudi Arabia (ERS). Several IBV outbreaks were recently …

Rapid metagenomic identification of two major swine pathogens with real-time Nanopore sequencing

L Chen, X Gao, W Xue, S Yuan, M Liu, Z Sun - Journal of Virological …, 2022 - Elsevier
Metagenomic next-generation sequencing (mNGS) is a rapid deep-sequencing diagnostic
tool for the unbiased identification of pathogens. In this study, we established a nanopore …

Special issue on applied next-generation sequencing in veterinary diagnostic laboratories

L Goodman, K Lahmers - Journal of Veterinary Diagnostic …, 2021 - journals.sagepub.com
The tools used in sequencing the human genome have been classified as next-generation
sequencing (NGS), a method of massively parallel, high-throughput sequencing that …

Viral Detection and Characterization in Free-Ranging Aquatic Turtles in the United States

W Laovechprasit - 2024 - search.proquest.com
Aquatic turtles, many of which are threatened or endangered, face numerous threats,
including anthropogenic interaction, ocean pollution, climate change, and infectious agents …

Advancements in Molecular Diagnostics for Detection of Mycoplasma gallisepticum and Mycoplasma synoviae in Poultry

RL Jude - 2021 - search.proquest.com
Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) are economically relevant
respiratory pathogens of poultry which may have analogous clinical presentations to …