How low can you go? Short-read polishing of Oxford Nanopore bacterial genome assemblies

G Bouras, LM Judd, RA Edwards… - Microbial …, 2024 - microbiologyresearch.org
It is now possible to assemble near-perfect bacterial genomes using Oxford Nanopore
Technologies (ONT) long reads, but short-read polishing is usually required for perfection …

Complementary insights into gut viral genomes: a comparative benchmark of short-and long-read metagenomes using diverse assemblers and binners

H Wang, C Sun, Y Li, J Chen, XM Zhao, WH Chen - Microbiome, 2024 - Springer
Background Metagenome-assembled viral genomes have significantly advanced the
discovery and characterization of the human gut virome. However, we lack a comparative …

Insights Into Phylogeny, Diversity and Functional Potential of Poseidoniales Viruses

A Prabhu, J Zaugg, CX Chan… - Environmental …, 2025 - Wiley Online Library
Viruses infecting archaea play significant ecological roles in marine ecosystems through
host infection and lysis, yet they have remained an underexplored component of the …

[PDF][PDF] Assessing Bias and Reproducibility of Viral Metagenomics Methods for the Combined Detection of Faecal RNA and DNA Viruses

R Haagmans, OJ Charity, D Baker, A Telatin… - 2024 - preprints.org
Whole transcriptome amplification (WTA2) and sequence-independent single primer
amplification (SISPA) are two widely used methods for combined metagenomic sequencing …

Missing the forest for the trees: time to address stool microbial load and everything else that realistically matters in microbiome studies

TT Oommen, R Ahamed… - Journal of Hepatology, 2024 - journal-of-hepatology.eu
The role of oral (represented by salivary microbiota), skin, gastrointestinal mucosal, and
stool microbiota in cirrhosis and their associations with disease severity and portal …

Limited consensus of marine viral diversity observed across techniques

M Martinez-Garcia, F Martinez-Hernandez, ML Gomez… - 2024 - researchsquare.com
Viruses are fundamental to many aspects of life influencing ecosystem functions. Thelenses
we use for exploring the viral diversity have expanded, yet at the same time each has …

Bioinformatic approaches for the prediction of seafloor ecological state from Nanopore 16SrRNA gene data

M Philip - 2024 - nmbu.brage.unit.no
The increasing anthropogenic pressures, particularly from industries and activities like
intensive aquaculture affect marine benthic ecosystems. This context requires immediate …