Genome-editing technologies, such as CRISPR–Cas9, are promising for treating otherwise
incurable genetic diseases. Great progress has been made for ex vivo genome editing; …

The clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR-
associated protein (Cas) systems are efficient and versatile gene editing tools, which offer …

Recently, chimeric antigen receptor (CAR)-T cell therapy has shown great promise in
treating haematological malignancies,,,,,–. However, CAR-T cell therapy currently has …

Targeted insertion of large DNA fragments holds great potential for treating genetic
diseases. Prime editors can effectively insert short fragments (~ 44 bp) but not large ones …

CD8 T cells play essential roles in anti-tumor immune responses. Here, we performed
genome-scale CRISPR screens in CD8 T cells directly under cancer immunotherapy …

Though AsCas12a fills a crucial gap in the current genome editing toolbox, it exhibits
relatively poor editing efficiency, restricting its overall utility. Here we isolate an engineered …

The efficiency of targeted knock-in for cell therapeutic applications is generally low, and the
scale is limited. In this study, we developed CLASH, a system that enables high-efficiency …

High-efficiency nonviral CRISPR/Cas9-mediated gene editing of human T cells using plasmid
donor DNA | Journal of Experimental Medicine | Rockefeller University Press Skip to Main …

Regulatory T cells are critical for maintaining immune tolerance. Recent studies have
confirmed their therapeutic suppressive potential to modulate immune responses in organ …

Proteogenomic identification of translated small open reading frames has revealed
thousands of previously unannotated, largely uncharacterized microproteins, or …