Next-generation sequencing for the detection of microbial agents in avian clinical samples

CL Afonso, AM Afonso - Veterinary Sciences, 2023 - mdpi.com
Simple Summary Significant progress in next-generation sequencing (NGS) is positioning
this technology as a key tool to be utilized in clinical diagnosis of disease agents and/or for …

Non-Targeted RNA Sequencing: Towards the Development of Universal Clinical Diagnosis Methods for Human and Veterinary Infectious Diseases

S Spatz, CL Afonso - Veterinary Sciences, 2024 - mdpi.com
Simple Summary With advances in NGS technology, the high ratio of throughput/cost per
base has allowed research laboratories to develop diagnostic protocols that can detect …

Differential viral genome diversity of healthy and RSS-affected broiler flocks

J Kubacki, W Qi, C Fraefel - Microorganisms, 2022 - mdpi.com
The intestinal virus community contributes to health and disease. Runting and stunting
syndrome (RSS) is associated with enteric viruses and leads to economic losses in the …

A metagenomic investigation of the faecal RNA virome structure of asymptomatic chickens obtained from a commercial farm in Durban, KwaZulu-Natal province, South …

VC Nwokorogu, S Pillai, JE San, C Pillay, MM Nyaga… - BMC genomics, 2024 - Springer
Background Virome studies on birds, including chickens are relatively scarce, particularly
from the African continent. Despite the continuous evolution of RNA viruses and severe …

Intestinal microbiota diversity from broilers with runting and stunting syndrome performed by metagenomics

DH Lages da Silva, RL Marques da Silva… - Avian …, 2024 - Taylor & Francis
Runting and stunting syndrome (RSS) is an enteric viral disease in commercial poultry that
directly affects gut health; however, its influence on gut microbiota remains unknown. This …

Development of a fast and sensitive RT-qPCR assay based on SYBR® green for diagnostic and quantification of Avian Nephritis Virus (ANV) in chickens affected with …

A Loor-Giler, S Castillo-Reyes… - BMC Veterinary …, 2024 - Springer
Background Enteric viruses are among the most prominent etiological agents of Runting-
Stunting Syndrome (RSS). The Avian Nephritis Virus (ANV) is an astrovirus associated with …

Development of a Double-Antibody Sandwich ELISA Based on a Monoclonal Antibody against the Viral NS1 Protein for the Detection of Chicken Parvovirus

M Zhang, J Liao, Z Xie, Y Zhang, S Luo, M Li, L Xie… - Pathogens, 2024 - mdpi.com
Chicken parvovirus (ChPV) infection can cause runting-stunting syndrome (RSS) in
chickens. There is currently no commercially available vaccine for controlling ChPV, and …

Investigation of avian rotavirus infections in broiler chicks from commercial flocks with different performance efficiency indexes

JC Gallego, D Lorencena, JL de Mello… - Veterinary Research …, 2022 - Springer
The aim of this study was to investigate and compare the frequency of occurrence of avian
rotavirus (AvRV) in poultry flocks according to its Performance Efficiency Index (PEI) scores …

Runting stunting syndrome in broiler chickens is associated with altered intestinal stem cell morphology and gene expression

SE Cloft, SR Kinstler, KE Reno, HS Sellers… - Avian …, 2022 - meridian.allenpress.com
Runting stunting syndrome (RSS) in broiler chickens is characterized by altered intestinal
morphology and gene expression and stunted growth. The objective of this study was to …

Advanced Diagnostics

LHM Miranda, M Gimeno, SL Donahoe… - Pathology of Pet and …, 2024 - Wiley Online Library
This chapter presents an overview of the different diagnostic techniques (special stains,
immunohistochemistry, in situ hybridization, and molecular diagnostics) available to the …