Advances in molecular genetic systems in malaria

TF de Koning-Ward, PR Gilson, BS Crabb - Nature Reviews …, 2015 - nature.com
Robust tools for analysing gene function in Plasmodium parasites, which are the causative
agents of malaria, are being developed at an accelerating rate. Two decades after genetic …

Enlightening the malaria parasite life cycle: bioluminescent Plasmodium in fundamental and applied research

G Siciliano, P Alano - Frontiers in microbiology, 2015 - frontiersin.org
The unicellular protozoan parasites of the genus Plasmodium impose on human health
worldwide the enormous burden of malaria. The possibility to genetically modify several …

A Plasmodium berghei reference line that constitutively expresses GFP at a high level throughout the complete life cycle

B Franke-Fayard, H Trueman, J Ramesar… - Molecular and …, 2004 - Elsevier
Green fluorescent protein (GFP) is a well-established reporter protein for the examination of
biological processes. This report describes a recombinant Plasmodium berghei …

Genetically attenuated, P36p-deficient malarial sporozoites induce protective immunity and apoptosis of infected liver cells

MR Van Dijk, B Douradinha… - Proceedings of the …, 2005 - National Acad Sciences
Immunization with Plasmodium sporozoites that have been attenuated by γ-irradiation or
specific genetic modification can induce protective immunity against subsequent malaria …

Molecular genetics and comparative genomics reveal RNAi is not functional in malaria parasites

J Baum, AT Papenfuss, GR Mair, CJ Janse… - Nucleic acids …, 2009 - academic.oup.com
Techniques for targeted genetic disruption in Plasmodium, the causative agent of malaria,
are currently intractable for those genes that are essential for blood stage development. The …

The development of genetic tools for dissecting the biology of malaria parasites

TF de Koning-Ward, CJ Janse… - Annual Reviews in …, 2000 - annualreviews.org
▪ Abstract Plasmodium parasites are haploid unicellular organisms that cause malaria. In the
last decade, transfection systems have been developed for both human and animal model …

Identification of regulatory elements in the Plasmodium falciparum genome

KT Militello, M Dodge, L Bethke, DF Wirth - Molecular and Biochemical …, 2004 - Elsevier
There is little information regarding regulatory sequences in the newly sequenced genome
of the malaria parasite, Plasmodium falciparum. Thus, for the first time, a bioinformatic …

The selectable marker human dihydrofolate reductase enables sequential genetic manipulation of the Plasmodium berghei genome

TF de Koning-Ward, DA Fidock, V Thathy… - Molecular and …, 2000 - Elsevier
Genetic transformation of malaria parasites has been limited by the number of selectable
markers available. For the rodent malaria parasite, Plasmodium berghei, only a single …

Clonally variant gene families in Plasmodium falciparum share a common activation factor

CA Howitt, D Wilinski, M Llinás… - Molecular …, 2009 - Wiley Online Library
The genome of the malaria parasite Plasmodium falciparum contains several multicopy
gene families, including var, rifin, stevor and Pfmc‐2TM. These gene families undergo …

Upregulation of expression of the reticulocyte homology gene 4 in the Plasmodium falciparum clone Dd2 is associated with a switch in the erythrocyte invasion …

D Gaur, T Furuya, J Mu, L Jiang, X Su… - Molecular and biochemical …, 2006 - Elsevier
The Plasmodium falciparum clone, Dd2, that requires sialic acid for invasion can switch to a
sialic acid independent pathway, Dd2 (NM). To elucidate the molecular basis of the switch in …