Advances in molecular genetic systems in malaria
Robust tools for analysing gene function in Plasmodium parasites, which are the causative
agents of malaria, are being developed at an accelerating rate. Two decades after genetic …
agents of malaria, are being developed at an accelerating rate. Two decades after genetic …
Enlightening the malaria parasite life cycle: bioluminescent Plasmodium in fundamental and applied research
G Siciliano, P Alano - Frontiers in microbiology, 2015 - frontiersin.org
The unicellular protozoan parasites of the genus Plasmodium impose on human health
worldwide the enormous burden of malaria. The possibility to genetically modify several …
worldwide the enormous burden of malaria. The possibility to genetically modify several …
A Plasmodium berghei reference line that constitutively expresses GFP at a high level throughout the complete life cycle
B Franke-Fayard, H Trueman, J Ramesar… - Molecular and …, 2004 - Elsevier
Green fluorescent protein (GFP) is a well-established reporter protein for the examination of
biological processes. This report describes a recombinant Plasmodium berghei …
biological processes. This report describes a recombinant Plasmodium berghei …
Genetically attenuated, P36p-deficient malarial sporozoites induce protective immunity and apoptosis of infected liver cells
MR Van Dijk, B Douradinha… - Proceedings of the …, 2005 - National Acad Sciences
Immunization with Plasmodium sporozoites that have been attenuated by γ-irradiation or
specific genetic modification can induce protective immunity against subsequent malaria …
specific genetic modification can induce protective immunity against subsequent malaria …
Molecular genetics and comparative genomics reveal RNAi is not functional in malaria parasites
Techniques for targeted genetic disruption in Plasmodium, the causative agent of malaria,
are currently intractable for those genes that are essential for blood stage development. The …
are currently intractable for those genes that are essential for blood stage development. The …
The development of genetic tools for dissecting the biology of malaria parasites
TF de Koning-Ward, CJ Janse… - Annual Reviews in …, 2000 - annualreviews.org
▪ Abstract Plasmodium parasites are haploid unicellular organisms that cause malaria. In the
last decade, transfection systems have been developed for both human and animal model …
last decade, transfection systems have been developed for both human and animal model …
Identification of regulatory elements in the Plasmodium falciparum genome
There is little information regarding regulatory sequences in the newly sequenced genome
of the malaria parasite, Plasmodium falciparum. Thus, for the first time, a bioinformatic …
of the malaria parasite, Plasmodium falciparum. Thus, for the first time, a bioinformatic …
The selectable marker human dihydrofolate reductase enables sequential genetic manipulation of the Plasmodium berghei genome
Genetic transformation of malaria parasites has been limited by the number of selectable
markers available. For the rodent malaria parasite, Plasmodium berghei, only a single …
markers available. For the rodent malaria parasite, Plasmodium berghei, only a single …
Clonally variant gene families in Plasmodium falciparum share a common activation factor
CA Howitt, D Wilinski, M Llinás… - Molecular …, 2009 - Wiley Online Library
The genome of the malaria parasite Plasmodium falciparum contains several multicopy
gene families, including var, rifin, stevor and Pfmc‐2TM. These gene families undergo …
gene families, including var, rifin, stevor and Pfmc‐2TM. These gene families undergo …
Upregulation of expression of the reticulocyte homology gene 4 in the Plasmodium falciparum clone Dd2 is associated with a switch in the erythrocyte invasion …
The Plasmodium falciparum clone, Dd2, that requires sialic acid for invasion can switch to a
sialic acid independent pathway, Dd2 (NM). To elucidate the molecular basis of the switch in …
sialic acid independent pathway, Dd2 (NM). To elucidate the molecular basis of the switch in …