Diabetes is a complex metabolic disorder of carbohydrate metabolism, characterized by
high blood glucose levels either due to an absolute deficiency of insulin secretion or an …

The multipotent pancreatic progenitor cells (MPCs) co-expressing the transcription factors,
PDX1 and NKX6. 1, are the source of functional pancreatic β-cells. The aim of this study was …

Remarkable advances in protocol development have been achieved to manufacture insulin-
secreting islets from human pluripotent stem cells (hPSCs). Distinct from current …

Cellular organelles play fundamental roles in almost all cell behaviors. Mitochondria have
been reported to be functionally linked to various biological processes, including …

Human hematopoietic stem cells (HSCs), which arise from aorta-gonad-mesonephros
(AGM), are widely used to treat blood diseases and cancers. However, a technique for their …

CRISPR systems have revolutionized biomedical research because they offer an
unprecedented opportunity for genome editing. However, a bottleneck of applying CRISPR …

Diabetes mellitus, a chronic and non-transmissible disease, triggers a wide range of micro-
and macrovascular complications. The differentiation of pancreatic β-like cells (PβLCs) from …

CRISPR/Cas-mediated genome editing in human pluripotent stem cells (hPSCs) offers
unprecedented opportunities for developing in vitro disease modeling, drug screening and …

Definitive endoderm (DE) derived from human pluripotent stem cells (hPSCs) holds great
promise for cell-based therapies and drug discovery. However, current DE differentiation …

Precise insertion of fluorescent proteins into lineage-specific genes in human pluripotent
stem cells (hPSCs) presents challenges due to low knockin efficiency and difficulties in …