Directed evolution: methodologies and applications

Y Wang, P Xue, M Cao, T Yu, ST Lane… - Chemical reviews, 2021 - ACS Publications
Directed evolution aims to expedite the natural evolution process of biological molecules
and systems in a test tube through iterative rounds of gene diversifications and library …

Physiological limitations and opportunities in microbial metabolic engineering

J Montaño López, L Duran, JL Avalos - Nature Reviews Microbiology, 2022 - nature.com
Metabolic engineering can have a pivotal role in increasing the environmental sustainability
of the transportation and chemical manufacturing sectors. The field has already developed …

Reprogramming methanol utilization pathways to convert Saccharomyces cerevisiae to a synthetic methylotroph

C Zhan, X Li, G Lan, EEK Baidoo, Y Yang, Y Liu… - Nature Catalysis, 2023 - nature.com
Methanol, an organic one-carbon (C1) compound, represents an attractive alternative
carbon source for microbial fermentation. Despite considerable advancements in methanol …

State-of-the-art technologies for biofuel production from triglycerides: A review

F Long, W Liu, X Jiang, Q Zhai, X Cao, J Jiang… - … and Sustainable Energy …, 2021 - Elsevier
Investigations and applications of renewable and sustainable energy have become central
for addressing the issue of emissions of greenhouse gases from the use of fossil …

Metabolic reconfiguration enables synthetic reductive metabolism in yeast

T Yu, Q Liu, X Wang, X Liu, Y Chen, J Nielsen - Nature Metabolism, 2022 - nature.com
Cell proliferation requires the integration of catabolic processes to provide energy, redox
power and biosynthetic precursors. Here we show how the combination of rational design …

[HTML][HTML] Recent advances in microbial production of medium chain fatty acid from renewable carbon resources: A comprehensive review

JH Ahn, KH Jung, ES Lim, SM Kim, SO Han… - Bioresource technology, 2023 - Elsevier
Microbial production of medium chain length fatty acids (MCFAs) from renewable resources
is becoming increasingly important in establishing a sustainable and clean chemical …

[HTML][HTML] Yeast synthetic biology advances biofuel production

Z Liu, J Wang, J Nielsen - Current Opinion in Microbiology, 2022 - Elsevier
Highlights•Biofuels represent valuable alternatives to fossil fuels to reduce net CO 2
emission.•S. cerevisiae is a widely used chassis with many available synthetic biology …

Electricity-driven microbial metabolism of carbon and nitrogen: a waste-to-resource solution

N Chu, Y Jiang, Q Liang, P Liu, D Wang… - Environmental …, 2023 - ACS Publications
Electricity-driven microbial metabolism relies on the extracellular electron transfer (EET)
process between microbes and electrodes and provides promise for resource recovery from …

Flux regulation through glycolysis and respiration is balanced by inositol pyrophosphates in yeast

N Qin, L Li, X Ji, R Pereira, Y Chen, S Yin, C Li, X Wan… - Cell, 2023 - cell.com
Although many prokaryotes have glycolysis alternatives, it's considered as the only energy-
generating glucose catabolic pathway in eukaryotes. Here, we managed to create a hybrid …

Coupling cell growth and biochemical pathway induction in Saccharomyces cerevisiae for production of (+)-valencene and its chemical conversion to (+)-nootkatone

Z Ye, Y Huang, B Shi, Z Xiang, Z Tian, M Huang… - Metabolic …, 2022 - Elsevier
Nootkatone is a valuable, functional sesquiterpene that is widely used in food, cosmetics,
pharmaceutical, agriculture, and other fields. However, only traces of it accumulate in plants …