Protein fusion tags are indispensible tools used to improve recombinant protein expression
yields, enable protein purification, and accelerate the characterization of protein structure …

In response to the rapidly growing field of proteomics, the use of recombinant proteins has
increased greatly in recent years. Recombinant hybrids containing a polypeptide fusion …

Perturbed cellular protein homeostasis (proteostasis) and mitochondrial dysfunction play an
important role in neurodegenerative diseases, however, the interplay between these two …

The TMEM16 family of proteins, also known as anoctamins, features a remarkable functional
diversity. This family contains the long sought-after Ca2+-activated chloride channels as well …

Transforming growth factor (TGF)-β is stored in the extracellular matrix as a latent complex
with its prodomain. Activation of TGF-β1 requires the binding of αv integrin to an RGD …

To dissect secretory traffic, we developed the retention using selective hooks (RUSH)
system. RUSH is a two-state assay based on the reversible interaction of a hook protein …

The ability to express or deplete proteins in living cells is crucial for the study of biological
processes. Viral vectors are often useful to deliver DNA constructs to cells that are difficult to …

Chromatin modifications are associated with many aspects of gene expression, yet their role
in cellular transitions during development remains elusive. Here, we use a new approach to …

The C-type lectin dectin-1 binds to yeast and signals through the kinase Syk and the adaptor
CARD9 to induce production of interleukin 10 (IL-10) and IL-2 in dendritic cells (DCs) …

Volume-regulated anion channels are activated in response to hypotonic stress. These
channels are composed of closely related paralogues of the leucine-rich repeat-containing …