Heterologous protein expression in the methylotrophic yeast Pichia pastoris

JL Cereghino, JM Cregg - FEMS microbiology reviews, 2000 - academic.oup.com
During the past 15 years, the methylotrophic yeast Pichia pastoris has developed into a
highly successful system for the production of a variety of heterologous proteins. The …

Recombinant protein expression in Pichia pastoris

JM Cregg, JL Cereghino, J Shi, DR Higgins - Molecular biotechnology, 2000 - Springer
The methylotrophic yeast Pichia pastoris is now one of the standard tools used in molecular
biology for the generation of recombinant protein. P. pastoris has demonstrated its most …

Molecular Healing of Polymeric Materials, Coatings, Plastics, Elastomers, Composites, Laminates, Adhesives, and Sealants by Active Enzymes

CS McDaniel, ME Wales, J Rawlins, P Cipi… - US Patent App. 12 …, 2010 - Google Patents
2010-04-30 Assigned to REACTIVE SURFACES, LTD. reassignment REACTIVE
SURFACES, LTD. ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR …

Optimization of the high-level production of Rhizopus oryzae lipase in Pichia pastoris

S Minning, A Serrano, P Ferrer, C Solá… - Journal of …, 2001 - Elsevier
The lipases of the Rhizopus species family are important and versatile enzymes that are
mainly used in fat and oil modification due to their strong 1, 3-regiospecificity. Inexpensive …

Expression of hepatitis B surface antigen in the methylotrophic yeast Pichia pastoris using the GAP promoter

A Vassileva, DA Chugh, S Swaminathan… - Journal of …, 2001 - Elsevier
High-level expression and efficient assembly of Hepatitis B surface Antigen (HBsAg)
particles have been reported in Pichia pastoris by integrating a single copy of the HBsAg …

Polymeric Coatings Incorporating Bioactive Enzymes for Cleaning a Surface

M Wales, CS McDaniel - US Patent App. 13/069,864, 2011 - Google Patents
C09D—COATING COMPOSITIONS, eg PAINTS, VARNISHES OR LACQUERS; FILLING
PASTES; CHEMICAL PAINT OR INK REMOVERS; INKS; CORRECTING FLUIDS; …

Visual Assays for Coatings Incorporating Bioactive Enzymes for Catalytic Functions

EB Williams, D Braasch, JW Rawlins… - US Patent App. 13 …, 2011 - Google Patents
Disclosed herein are materials such as a coating, comprising a lipolytic enzyme or
organophosphrous compound degrading enzyme. Also disclosed herein are methods of …

Coatings and Surface Treatments Having Active Enzymes and Peptides

CS McDaniel - US Patent App. 12/474,921, 2010 - Google Patents
Disclosed herein are a materials such as a coating, an elastomer, an adhesive, a sealant, a
textile finish, a wax, and a filler for such a material, wherein the material includes an enzyme …

Expression in Pichia pastoris of Candida antarctica lipase B and lipase B fused to a cellulose-binding domain

JC Rotticci-Mulder, M Gustavsson, M Holmquist… - Protein expression and …, 2001 - Elsevier
Candida antarctica lipase B (CALB) and C. antarctica lipase B fused to a cellulose-binding
domain (CBD-CALB) were expressed functionally in the methylotrophic yeast Pichia …

Anti-fouling Paints and Coatings

CS McDaniel - US Patent App. 14/097,128, 2015 - Google Patents
Disclosed herein are polymeric materials such as a coating, a plastic, a laminate, a
composite, an elastomer, an adhesive, or a sealant; a surface treatment such as a textile …