Isothermal titration calorimetry is a widely used biophysical technique for studying the
formation or dissociation of molecular complexes. Over the last 5 years, much work has …

Cytochrome P450 enzymes catalyze a broad diversity of reactions, including well-
established transformations such as carbon and nitrogen hydroxylation, heteroatom …

Background Enzyme active sites can be connected to the exterior environment by one or
more channels passing through the protein. Despite our current knowledge of enzyme …

We used high hydrostatic pressure as a tool for exploring the conformational landscape of
human cytochrome P450 3A4 (CYP3A4) by electron paramagnetic resonance and …

A proper description of proton donor–acceptor (D–A) distance fluctuations is crucial for
understanding tunneling in proton-coupled electron transport (PCET). The typical harmonic …

Mycobacteria share a common cholesterol degradation pathway initiated by oxidation of the
alkyl side chain by enzymes of cytochrome P450 (CYP) families 125 and 142. Structural and …

The complex multistep mechanism of oxygen activation in P450 is reviewed as a sequence
of the following reactions: Substrate binding, reduction of the heme iron from ferric to the …

Biological routes to the production of fuels from renewable feedstocks hold significant
promise in our efforts towards a sustainable future. The fatty acid decarboxylase enzyme …

Inhibition of bacterial nitric oxide synthase (bNOS) has the potential to improve the efficacy
of antimicrobials used to treat infections by Gram-positive pathogens Staphylococcus aureus …

Efficiently carrying out the oxygen reduction reaction (ORR) is critical for many applications
in biology and chemistry, such as bioenergetics and fuel cells, respectively. In biology, this …