Rapid changes of protein phosphorylation play a crucial role in the regulation of many
cellular processes. Being post-translationally modified, phosphoproteins are often present in …

Phosphorylation is a form of protein posttranslational modification (PTM) that regulates many
biological processes. Whereas phosphoproteomics is a scientific discipline that identifies …

Shotgun proteomics dominates the field of proteomics. The foundations of the strategy
consist of multiple rounds of peptide separation where chromatography provides the …

Phosphoproteomics is increasingly used to address a wide range of biological questions.
However, despite some success, techniques for phosphoproteomics are not without …

Most phosphoproteomics experiments rely on prefractionation of tryptic digests before online
liquid chromatography-mass spectrometry. This study compares the potential and limitations …

Trypsin, Lys-C, and Lys-N are the most broadly used enzymes in proteomics. Here, on the
basis of large-scale peptide mass spectrometry (MS) data sets, an approach is described to …

Pituitary adenoma (PA) is a common intracranial neoplasm, which affects the hypothalamus-
pituitary-target organ axis systems, and is hazardous to human health. Post-translational …

Phosphopeptides play a crucial role in many biological processes and constitute some of
the most powerful biomarkers in disease detection. However they are often present in very …

The proteins in royal jelly (RJ) play a pivotal role in the nutrition, immune defense, and cast
determination of honeybee larvae and have a wide range of pharmacological and health …

This report examines the analytical benefits of high-field asymmetric waveform ion mobility
spectrometry (FAIMS) coupled to liquid chromatography mass spectrometry (LC–MS) for …