Nearly 80% of the approved human therapeutic antibodies are produced by Chinese
Hamster Ovary (CHO) cells. To achieve better cell growth and high-yield recombinant …

At present, nearly 70% of recombinant therapeutic proteins (RTPs) are produced by Chinese
hamster ovary (CHO) cells, and serum-free medium (SFM) is necessary for their culture to …

Highlights•Sensing is critical to achieve optimal productivity and product quality.•Monitoring
biomass and substrate levels relates directly to productivity and quality.•A major challenge is …

The N-linked glycosylation pattern is an important quality attribute of therapeutic
glycoproteins. It has been reported by our group and by others that different carbon sources …

A novel approach of design of experiment (DoE) is developed for the optimization of key
substrates of the culture medium, amino acids, and sugars, by utilizing perfusion …

Chinese hamster ovary (CHO) cells can produce proteins with complex structures and post-
translational modifications which are similar to human-derived cells, and they have been the …

Multivariate latent variable methods have become a popular and versatile toolset to analyze
bioprocess data in industry and academia. This work spans such applications from the …

The rapidly growing, competitive biopharmaceutical market requires tight bioprocess
monitoring. An integrated, automated platform for the routine online/at‐line monitoring of key …

Background Raman spectroscopy has gained popularity to monitor multiple process
indicators simultaneously in biopharmaceutical processes. However, robust and specific …

The variation of antigen binding sites on monoclonal antibodies (mAbs) enables
manufacturing of the latter for targeted treatment of a variety of ailments, including cancer …