Streptavidin–biotin technology: improvements and innovations in chemical and biological applications
Streptavidin and its homologs (together referred to as streptavidin) are widely used in
molecular science owing to their highly selective and stable interaction with biotin. Other …
molecular science owing to their highly selective and stable interaction with biotin. Other …
Synthetic mimics of biotin/(strept) avidin
Biotin/(strept) avidin self-assembly is a powerful platform for nanoscale fabrication and
capture with many different applications in science, medicine, and nanotechnology …
capture with many different applications in science, medicine, and nanotechnology …
Peptide-encoding mRNA barcodes for the high-throughput in vivo screening of libraries of lipid nanoparticles for mRNA delivery
Developing safe and effective nanoparticles for the delivery of messenger RNA (mRNA) is
slow and expensive, partly due to the lack of predictive power of in vitro screening methods …
slow and expensive, partly due to the lack of predictive power of in vitro screening methods …
[HTML][HTML] Reprogramming macrophages via immune cell mobilized hydrogel microspheres for osteoarthritis treatments
Regulating macrophage activation precisely is crucial in treating chronic inflammation in
osteoarthritis (OA). However, the stable pro-inflammatory state and deep distribution of …
osteoarthritis (OA). However, the stable pro-inflammatory state and deep distribution of …
Cell type-specific delivery by modular envelope design
The delivery of genetic cargo remains one of the largest obstacles to the successful
translation of experimental therapies, in large part due to the absence of targetable delivery …
translation of experimental therapies, in large part due to the absence of targetable delivery …
Efficient generation of targeted large insertions by microinjection into two-cell-stage mouse embryos
Rapid, efficient generation of knock-in mice with targeted large insertions remains a major
hurdle in mouse genetics. Here, we describe two-cell homologous recombination (2C-HR) …
hurdle in mouse genetics. Here, we describe two-cell homologous recombination (2C-HR) …
Site-specific biotinylation of purified proteins using BirA
M Fairhead, M Howarth - Site-Specific Protein Labeling: Methods and …, 2015 - Springer
The binding between biotin and streptavidin or avidin is one of the strongest known non-
covalent biological interactions. The (strept) avidin-biotin interaction has been widely used …
covalent biological interactions. The (strept) avidin-biotin interaction has been widely used …
BacPROTACs mediate targeted protein degradation in bacteria
FE Morreale, S Kleine, J Leodolter, S Junker, DM Hoi… - Cell, 2022 - cell.com
Hijacking the cellular protein degradation system offers unique opportunities for drug
discovery, as exemplified by proteolysis-targeting chimeras. Despite their great promise for …
discovery, as exemplified by proteolysis-targeting chimeras. Despite their great promise for …
Genetically targeted chemical assembly of polymers specifically localized extracellularly to surface membranes of living neurons
Multicellular biological systems, particularly living neural networks, exhibit highly complex
organization properties that pose difficulties for building cell-specific biocompatible …
organization properties that pose difficulties for building cell-specific biocompatible …
Site-specific protein labeling and generation of defined ubiquitin-protein conjugates using an asparaginyl endopeptidase
Asparaginyl endopeptidases (AEPs) have recently been widely utilized for peptide and
protein modification. Labeling is however restricted to protein termini, severely limiting …
protein modification. Labeling is however restricted to protein termini, severely limiting …