Aptamer-based biosensors for biomedical diagnostics
Aptamers are single-stranded nucleic acids that selectively bind to target molecules. Most
aptamers are obtained through a combinatorial biology technique called SELEX. Since …
aptamers are obtained through a combinatorial biology technique called SELEX. Since …
Nucleic acid ligands with protein-like side chains: modified aptamers and their use as diagnostic and therapeutic agents
JC Rohloff, AD Gelinas, TC Jarvis, UA Ochsner… - … Therapy-Nucleic Acids, 2014 - cell.com
Limited chemical diversity of nucleic acid libraries has long been suspected to be a major
constraining factor in the overall success of SELEX (Systematic Evolution of Ligands by …
constraining factor in the overall success of SELEX (Systematic Evolution of Ligands by …
Functional nucleic acid sensors
Sensors are devices that respond to physical or chemical stimuli and produce detectable
signals. 1r4 They are a critical extension of human perception of the world in many aspects …
signals. 1r4 They are a critical extension of human perception of the world in many aspects …
Aptamers: an emerging class of molecules that rival antibodies in diagnostics
SD Jayasena - Clinical chemistry, 1999 - academic.oup.com
Antibodies, the most popular class of molecules providing molecular recognition needs for a
wide range of applications, have been around for more than three decades. As a result …
wide range of applications, have been around for more than three decades. As a result …
Microfluidic designs and techniques using lab-on-a-chip devices for pathogen detection for point-of-care diagnostics
Effective pathogen detection is an essential prerequisite for the prevention and treatment of
infectious diseases. Despite recent advances in biosensors, infectious diseases remain a …
infectious diseases. Despite recent advances in biosensors, infectious diseases remain a …
SELEX—A (r) evolutionary method to generate high-affinity nucleic acid ligands
R Stoltenburg, C Reinemann, B Strehlitz - Biomolecular engineering, 2007 - Elsevier
SELEX stands for systematic evolution of ligands by exponential enrichment. This method,
described primarily in 1990 [Ellington, AD, Szostak, JW, 1990. In vitro selection of RNA …
described primarily in 1990 [Ellington, AD, Szostak, JW, 1990. In vitro selection of RNA …
Adaptive recognition by nucleic acid aptamers
Nucleic acid molecules play crucial roles in diverse biological processes including the
storage, transport, processing, and expression of the genetic information. Nucleic acid …
storage, transport, processing, and expression of the genetic information. Nucleic acid …
RNA-peptide fusions for the in vitro selection of peptides and proteins
RW Roberts, JW Szostak - Proceedings of the National …, 1997 - National Acad Sciences
Covalent fusions between an mRNA and the peptide or protein that it encodes can be
generated by in vitro translation of synthetic mRNAs that carry puromycin, a peptidyl …
generated by in vitro translation of synthetic mRNAs that carry puromycin, a peptidyl …
Thiamine derivatives bind messenger RNAs directly to regulate bacterial gene expression
W Winkler, A Nahvi, RR Breaker - Nature, 2002 - nature.com
Although proteins fulfil most of the requirements that biology has for structural and functional
components such as enzymes and receptors, RNA can also serve in these capacities. For …
components such as enzymes and receptors, RNA can also serve in these capacities. For …
In vitro selection and evolution of functional proteins by using ribosome display
J Hanes, A Plückthun - … of the National Academy of Sciences, 1997 - National Acad Sciences
We report here a system with which a correctly folded complete protein and its encoding
mRNA both remain attached to the ribosome and can be enriched for the ligand-binding …
mRNA both remain attached to the ribosome and can be enriched for the ligand-binding …